Izvorni znanstveni članak
https://doi.org/10.5513/JCEA01/20.4.2294
The effect of kaempferol and naringenin may improve the in vitro quality of stored boar semen
Michal Duračka
orcid.org/0000-0002-2907-5909
; Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Slovak Republic
Megane Debacker
; Condorcet - Hainaut Provincial High School, Tournai, Belgium
Ondrej Bučko
; Department of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Slovak Republic
Norbert Lukač
; Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Slovak Republic
Eva Tvrda
; Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Slovak Republic
Sažetak
Determination of the effect of pure bioactive substances on male reproductive cells is a current theme amongst veterinary andrologists. The aim of this study was to investigate the in vitro effect of various concentrations of kaempferol and naringenin on boar male gametes in different time periods (0, 24, 48 and 72h). The computer-assisted semen analysis was used to measure the sperm motility. Mitochondrial activity was determined by the mitochondrial toxicity test (MTT). The formation of the superoxide radical, as a marker of oxidative stress, was analysed by the nitroblue-tetrazolium (NBT) test. The results showed that every selected concentration of both substances came along with beneficial effect on the structure and function of spermatozoa. Particularly, naringenin at concentrations of 10-50 µmol/L and kaempferol at concentrations of 5-25 µmol/L may provide beneficial effects on spermatozoa, thus prolong the storage period and improve the quality of preserved boar spermatozoa.
Ključne riječi
antioxidants; bioactive compounds; kaempferol; naringenin; sperm; spermatozoa; oxidative stress; reactive oxygen species; mitochondrial activity
Hrčak ID:
230867
URI
Datum izdavanja:
19.12.2019.
Posjeta: 1.560 *