Skoči na glavni sadržaj

Izvorni znanstveni članak

https://doi.org/10.15255/CABEQ.2019.1699

TiO2 Sol-gel Coating as a Transducer Substrate for Impedimetric Immunosensors

R. A. D. de Faria orcid id orcid.org/0000-0002-7025-7042 ; Department of Chemical Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-90, Brazil
M. Houmard ; Department of Materials and Construction Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-90, Brazil
V. A. M. do Rosário ; Department of Applied Immunology, Fundação Ezequiel Dias, Belo Horizonte, MG 30510-010, Brazil
V. de Freitas Cunha Lins ; Department of Chemical Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-90, Brazil
L. G. D. Heneine ; Department of Applied Immunology, Fundação Ezequiel Dias, Belo Horizonte, MG 30510-010, Brazil
T. Matencio ; Department of Chemistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-90, Brazil


Puni tekst: engleski pdf 962 Kb

str. 437-447

preuzimanja: 207

citiraj


Sažetak

Given the importance of the transducer elements in the performance of sensors for various applications, as well as the growing search for devices that are capable of providing the response in shorter time, in this work, titanium dioxide was examined as a candidate for application in an electrochemical biosensor. A TiO2 coating deposited by sol-gel
method on a silicon wafer was obtained with an anatase crystalline structure, as an n-type semiconductor with donor density equal to 2.954 · 1017 cm–3. Its surface was functionalized to be tested as a biosensor to detect snake venom of the Bothrops genera, and each step of the functionalization was investigated using Electrochemical Impedance Spectroscopy (EIS) and Cyclic voltammetry. Despite being less sensitive than the reference method ELISA, the TiO2-based biosensor was also capable of detecting the analyte of interest at 20 μg mL–1, revealing an increase in its leakage resistance and phase shift after incubation in this solution. Furthermore, the total time for carrying out the biodetection
with the TiO2-coated device (41.24 ± 0.05 min) was estimated to be approximately 80 % shorter than that required by the labelled standard assay, which indicates that TiO2 is a promising electrochemical transducer for biosensing applications.







This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi

titanium dioxide, biosensor, transducer, Electrochemical Impedance Spectroscopy

Hrčak ID:

232512

URI

https://hrcak.srce.hr/232512

Posjeta: 370 *