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Genotoxicity of Fumonisin B1, Beauvericin and Ochratoxin A in Porcine Kidney PK15 Cells: Effects of Individual and Combined Treatment

Maja Šegvić Klarić ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Stjepan Pepeljnjak ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Ružica Rozgaj ; Institute for Medical Research and Occupational Health, Zagreb, Croatia

Puni tekst: engleski, pdf (235 KB) str. 139-146 preuzimanja: 629* citiraj
APA 6th Edition
Šegvić Klarić, M., Pepeljnjak, S. i Rozgaj, R. (2008). Genotoxicity of Fumonisin B1, Beauvericin and Ochratoxin A in Porcine Kidney PK15 Cells: Effects of Individual and Combined Treatment. Croatica Chemica Acta, 81 (1), 139-146. Preuzeto s https://hrcak.srce.hr/23438
MLA 8th Edition
Šegvić Klarić, Maja, et al. "Genotoxicity of Fumonisin B1, Beauvericin and Ochratoxin A in Porcine Kidney PK15 Cells: Effects of Individual and Combined Treatment." Croatica Chemica Acta, vol. 81, br. 1, 2008, str. 139-146. https://hrcak.srce.hr/23438. Citirano 15.09.2019.
Chicago 17th Edition
Šegvić Klarić, Maja, Stjepan Pepeljnjak i Ružica Rozgaj. "Genotoxicity of Fumonisin B1, Beauvericin and Ochratoxin A in Porcine Kidney PK15 Cells: Effects of Individual and Combined Treatment." Croatica Chemica Acta 81, br. 1 (2008): 139-146. https://hrcak.srce.hr/23438
Harvard
Šegvić Klarić, M., Pepeljnjak, S., i Rozgaj, R. (2008). 'Genotoxicity of Fumonisin B1, Beauvericin and Ochratoxin A in Porcine Kidney PK15 Cells: Effects of Individual and Combined Treatment', Croatica Chemica Acta, 81(1), str. 139-146. Preuzeto s: https://hrcak.srce.hr/23438 (Datum pristupa: 15.09.2019.)
Vancouver
Šegvić Klarić M, Pepeljnjak S, Rozgaj R. Genotoxicity of Fumonisin B1, Beauvericin and Ochratoxin A in Porcine Kidney PK15 Cells: Effects of Individual and Combined Treatment. Croatica Chemica Acta [Internet]. 2008 [pristupljeno 15.09.2019.];81(1):139-146. Dostupno na: https://hrcak.srce.hr/23438
IEEE
M. Šegvić Klarić, S. Pepeljnjak i R. Rozgaj, "Genotoxicity of Fumonisin B1, Beauvericin and Ochratoxin A in Porcine Kidney PK15 Cells: Effects of Individual and Combined Treatment", Croatica Chemica Acta, vol.81, br. 1, str. 139-146, 2008. [Online]. Dostupno na: https://hrcak.srce.hr/23438. [Citirano: 15.09.2019.]

Sažetak
After 24- and 48-hour treatment with 0.05, 0.5 and 5 mg/mL of fumonisin (FB1), beauvericin (BEA) and ochratoxin A (OTA) alone or combinations of two or all three mycotoxins in equal concentrations, Giemsa-stained binucleated PK15 cells were scored for the presence of micronuclei (MN), nuclear buds (NBs) and nucleoplasmic bridges (NPBs). Single mycotoxins induced MN and NPBs in a dose-dependent manner, showing predominantly clastogenic effect. OTA showed stronger genotoxic potential than FB1 and BEA, since 0.05 and 0.5 mg/mL OTA increased the frequency of MN and NPBs, respectively. Mycotoxin combinations increased the frequency of MN and NPBs, mostly in additive manner. A dose-dependent increase in NBs was observed after 24-hour exposure to single mycotoxins. Prolonged treatment with 5 mg/mL of each toxin alone as well as combined exposure to all mycotoxin concentrations resulted in a decrease in NBs, which could be related to the extrusion of micronuclei and/or saturation of the genotoxic effect. This is the first report on genotoxicity of BEA.

Ključne riječi
fumonisin B1; beauvericin; ochratoxin A; micronucleus; nuclear buds; nucleoplasmic bridges

Hrčak ID: 23438

URI
https://hrcak.srce.hr/23438

Posjeta: 919 *