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https://doi.org/10.5552/drvind.2020.1958

Optimal Surface Preparation for Wood Anatomy Research of Invasive Species by Scanning Electron Microscopy

Maks Merela   ORCID icon orcid.org/0000-0001-7927-2049 ; University of Ljubljana, Biotechnical Faculty, Department of Wood Science and Technology, Ljubljana, Slovenia
Nejc Thaler ; Sivaprodukt d.o.o., Ljubljana, Slovenia
Angela Balzano ; University of Ljubljana, Biotechnical Faculty, Department of Wood Science and Technology, Ljubljana, Slovenia
Denis Plavčak ; University of Ljubljana, Biotechnical Faculty, Department of Wood Science and Technology, Ljubljana, Slovenia

Puni tekst: engleski, pdf (2 MB) str. 117-127 preuzimanja: 215* citiraj
APA 6th Edition
Merela, M., Thaler, N., Balzano, A. i Plavčak, D. (2020). Optimal Surface Preparation for Wood Anatomy Research of Invasive Species by Scanning Electron Microscopy. Drvna industrija, 71 (2), 117-127. https://doi.org/10.5552/drvind.2020.1958
MLA 8th Edition
Merela, Maks, et al. "Optimal Surface Preparation for Wood Anatomy Research of Invasive Species by Scanning Electron Microscopy." Drvna industrija, vol. 71, br. 2, 2020, str. 117-127. https://doi.org/10.5552/drvind.2020.1958. Citirano 07.05.2021.
Chicago 17th Edition
Merela, Maks, Nejc Thaler, Angela Balzano i Denis Plavčak. "Optimal Surface Preparation for Wood Anatomy Research of Invasive Species by Scanning Electron Microscopy." Drvna industrija 71, br. 2 (2020): 117-127. https://doi.org/10.5552/drvind.2020.1958
Harvard
Merela, M., et al. (2020). 'Optimal Surface Preparation for Wood Anatomy Research of Invasive Species by Scanning Electron Microscopy', Drvna industrija, 71(2), str. 117-127. https://doi.org/10.5552/drvind.2020.1958
Vancouver
Merela M, Thaler N, Balzano A, Plavčak D. Optimal Surface Preparation for Wood Anatomy Research of Invasive Species by Scanning Electron Microscopy. Drvna industrija [Internet]. 2020 [pristupljeno 07.05.2021.];71(2):117-127. https://doi.org/10.5552/drvind.2020.1958
IEEE
M. Merela, N. Thaler, A. Balzano i D. Plavčak, "Optimal Surface Preparation for Wood Anatomy Research of Invasive Species by Scanning Electron Microscopy", Drvna industrija, vol.71, br. 2, str. 117-127, 2020. [Online]. https://doi.org/10.5552/drvind.2020.1958

Sažetak
Research was done to develop the optimal method of wood surface preparation for scanning electron microscopy (SEM). Since 2018, environmental scanning electron microscope (ESEM™) FEI Quanta 250 has been installed at the Department of Wood Science and Technology in Ljubljana. We tested several methods for the pre-preparation and cutting of wood surfaces for SEM analyses. The samples had been either dried, soaked in water and frozen, impregnated with paraffin or simply moistened before cutting. We analysed wood surfaces obtained by splitting, sawing, planing, sanding and cutting on a sliding microtome with different blades. The effect of gold coating on the SEM image quality was also evaluated. Best results were obtained by cutting a pre-moistened surface on a sliding microtome with a low profile replaceable blade and gold coated afterwards. Determined methodology is technically less demanding, not time consuming and obtains results that satisfy needs for wood anatomy research at magnifications up to 12.000x. Guidelines for the optimal preparation of samples were prepared, and theoretical and practical basis for investigations of wood anatomy using SEM were provided. The method was afterwards used in analyses of invasive alien plant species – investigating their anatomical structure in the framework of the AlienPLAntSpEcies - APPLAUSE project (Urban Innovative Actions initiative). It was demonstrated that the use of the SEM opened new scope in detailed investigations of the wood structure and properties.

Ključne riječi
wood anatomy; wood surface; scanning electron microscopy; SEM; invasive species; sample preparation

Hrčak ID: 238920

URI
https://hrcak.srce.hr/238920

[hrvatski]

Posjeta: 495 *