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https://doi.org/10.2478/aiht-2020-71-3399

Superior protective effects of in vitro propagated green garlic against hydrogen peroxide-induced cytotoxicity in human hepatoma cells

Tomislav Vinković ; Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, Croatia
Nada Parađiković ; Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, Croatia
Monika Tkalec Kojić ; Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, Croatia
Gordana Mendaš ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Tanja Živković Semren ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Valentina Gluščić ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ivana Vinković Vrček ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ivan Pavičić orcid id orcid.org/0000-0003-2697-6514 ; Institute for Medical Research and Occupational Health, Zagreb, Croatia


Puni tekst: engleski pdf 361 Kb

str. 130-137

preuzimanja: 416

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Sažetak

Garlic is a valuable source material for medicines due to its known antitumor, hypolipidaemic, antioxidant, and immunomodulatory effects. This study compares the protective effects of conventionally grown (CG) and in vitro propagated garlic (PG) against hydrogen peroxide-induced cytotoxicity in HepG2 cells and their antioxidant activity. Garlic used in this study was obtained by planting garlic cloves or by planting the transplants of PG directly in the field. At the end of the vegetation period, CG and PG were sampled and extracts prepared for the experiment. Compared to conventionally grown garlic bulbs, PG leafy part yielded significantly higher content of polyphenols, flavonoids and alliin, and also showed equal or higher antioxidant activity, measured by the cell viability test, GSH and ROS level. Moreover, PG can be produced in less time (shorter vegetation period) and with significantly less material (cloves). Significantly higher content of alliin, polyphenols, and flavonoids and significantly higher yield of plant biomass in PG has a great potential to become a new production model with improved garlic properties as a medicine material.

Ključne riječi

Allium sativum L., alliin, antioxidant activity, glutathione, total phenolic content, total flavonoid content

Hrčak ID:

239249

URI

https://hrcak.srce.hr/239249

Podaci na drugim jezicima: hrvatski

Posjeta: 771 *