Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2019.60.439
Adipose-derived mesenchymal stem cells attenuate ischemic brain injuries in rats by modulating miR-21-3p/MAT2B signaling transduction
Chunshuang Li
; Department of Emergency Longnan Hospital of Daqing, Daqing, China
Kexin Fei
; Department of Emergency Longnan Hospital of Daqing, Daqing, China
Feipeng Tian
; Department of Bone Surgery, Longnan Hospital of Daqing, Daqing, China
Feipeng Tian
; Department of Bone Surgery, Longnan Hospital of Daqing, Daqing, China
Changkui Gao
; Department of Emergency Longnan Hospital of Daqing, Daqing, China
Yang Song
; Department of Emergency Longnan Hospital of Daqing, Daqing, China
Sažetak
Aim To explore the mechanism underlying the protective
effect of adipose-derived mesenchymal stem cells (ADMSCs)
against ischemic stroke by focusing on miR-21-3p/
MAT2B axis.
Methods Ischemic brain injury was induced in 126 rats
by middle cerebral artery occlusion (MCAO). The effect of
ADMSC administration on blood-brain barrier (BBB) condition,
apoptosis, inflammation, and the activity of miR-21-
3p/MAT2B axis was assessed. The role of miR-21-3p inhibition
in the function of ADMSCs was further validated in in
vitro neural cells.
Results ADMSCs administration improved BBB condition,
inhibited apoptosis, and suppressed inflammation. It also
reduced the abnormally high level of miR-21-3p in MCAO
rats. Dual luciferase assays showed that miR-21-3p directly
inhibited the MAT2B expression in neural cells, and miR-21
-3p inhibition by inhibitor or ADMSC-derived exosomes in
neurons attenuated hypoxia/reoxygenation-induced impairments
similarly to that of ADMSCs in vivo.
Conclusion This study confirmed the protective effect
of ADMSCs against ischemic brain injury exerted by suppressing
miR-21-3p level and up-regulating MAT2B level.
Ključne riječi
Hrčak ID:
240108
URI
Datum izdavanja:
15.10.2019.
Posjeta: 742 *