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https://doi.org/10.2478/aiht-2020-71-3442

Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii

Dolores Peruč ; University of Rijeka Faculty of Medicine, Department of Microbiology and Parasitology, Rijeka, Croatia
Brigita Tićac ; University of Rijeka Faculty of Medicine, Department of Microbiology and Parasitology, Rijeka, Croatia
Dalibor Broznić ; University of Rijeka Faculty of Medicine, Department of Medical Chemistry, Biochemistry and Clinical Chemistry, Rijeka, Croatia
Ivana Gobin ; University of Rijeka Faculty of Medicine, Department of Microbiology and Parasitology, Rijeka, Croatia


Puni tekst: engleski pdf 635 Kb

verzije

str. 223-230

preuzimanja: 513

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Sažetak

Acanthamoeba is an opportunistic protozoon, widespread in the aquatic environment, where it can be in endosymbiosis with over 30 pathogenic bacteria, including nontuberculous mycobacteria (NTM). Protozoa play a crucial role in mycobacterial pathogenesis and serve as a reservoir of infection. Since the first step in bacteria making contact with amoebae is adhesion, we were interested in investigating whether essential oils (EOs) can affect it. To that end we investigated the effects of juniper (Juniperus communis) and immortelle (Helichrysum italicum) EOs against Mycobacterium avium, M. intracellulare, and M. gordonae in tap water and against their adhesion to Acanthamoeba castellanii by combining them in synergistic EO concentrations. M. avium and M. intracellulare adhered to A. castellanii to a greater extent than M. gordonae. The adhesion of all NTMs was prevented by the subinhibitory concentrations of EOs. When comparing the effect of synergistic combinations of EOs and the effect of a single concentration from a combination, a higher percentage of adhesion inhibition in all synergistic combinations observed, except against M. gordonae. Neither oil was cytotoxic to A. castellanii. Our findings suggest that the EOs or their components weaken the contact of environmental NTMs and free-living amoebae and indirectly diminish their pathogenic potential, which could be of value in developing strategies for maintenance of water supply systems.

Ključne riječi

Acanthamoeba castellanii; antiadhesion; Helichrysum italicum; Juniperus communis; Mycobacterium avium; Mycobacterium gordonae; Mycobacterium intracellulare

Hrčak ID:

243872

URI

https://hrcak.srce.hr/243872

Datum izdavanja:

22.9.2020.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.396 *