Veterinarski arhiv, Vol. 77 No. 4, 2007.
Izvorni znanstveni članak
Molecular detection of Ehrlichia canis from blood of naturally infected dogs in India
Bindu Lakshmanan
; Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Wayanad, India
Lalitha John
; Dean, Madras Veterinary College, Chennai, India
Sankaralingam Gomathinayagam
; Department of Parasitology, Madras Veterinary College, Chennai, India
Gopal Dhinakarraj
; Department of Animal Biotechnology, Madras Veterinary College, Chennai, India
Sažetak
The nested PCR assay for the detection of 16S rRNA gene fragment of Ehrlichia canis from the blood of dogs was standardized for the first time in India. The genus-specific primers amplified a 477 bp band of Ehrlichia sp. in the first PCR. The nested PCR assay using species-specific primers produced a 387 bp band of E. canis. The nested PCR detected E. canis organisms in 50% of samples as against the routine blood smear examination, which revealed morulae in only 19.38% of samples. This protocol could detect the presence of E. canis, even one month after specific antibiotic therapy.
Ključne riječi
Ehrlichia canis; nested PCR; 16S rRNA gene; dogs
Hrčak ID:
24770
URI
Datum izdavanja:
20.8.2007.
Posjeta: 1.597 *