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Veterinary Archives, Vol. 77 No. 2, 2007.

Original scientific paper

Characterization of leptospiral isolates using random amplified polymorphic DNA (RAPD) fingerprinting

Isam El Jalii orcid id orcid.org/0000-0002-5793-5873 ; Department of Preventive Medicine and Public Health, Faculty of Veterinary Medicine, University of Khartoum, Khartoum North, Sudan
Abdul Rani Bahaman ; Faculty of Veterinary Medicine, University Putra Malaysia, Serdang, Selangor, Malaysia

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Abstract

Random amplified polymorphic DNA (RAPD) fingerprinting was applied to characterize leptospiral strains. Two primers were used to generate individual RAPD fingerprints. The DNA fingerprints obtained were distinct and reproducible. The fingerprints obtained could be useful for distinguishing and characterizing the strains. Profiles obtained revealed genetic heterogeneity between strains belonging to one serovar. In conclusion, different DNA fingerprints were obtained which would allow characterization of the strains.

Keywords

RAPD; leptospira; serovar; characterization

Hrčak ID:

25094

URI

https://hrcak.srce.hr/25094

Publication date:

20.4.2007.

Article data in other languages: croatian

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