Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2020.1834
Preparation and Characterization of Electrospun PCL/Silk Fibroin Scaffolds
E. Govorčin Bajsić
; University of Zagreb, Faculty of Chemical Engineering and Technology, Marulićev trg 19, 10000 Zagreb, Croatia
E. Zdraveva
; University of Zagreb, Faculty of Textile Technology, Prilaz baruna Filipovića 28a, 10000 Zagreb, Croatia
T. Holjevac Grgurić
; University of Zagreb, Faculty of Metallurgy, Aleja narodnih heroja 3, 44000 Sisak, Croatia
I. Slivac
; University of Zagreb, Faculty of Food Technology and Biotechnology, Pierottijeva ul. 6, 10000 Zagreb, Croatia
M. Tominac Trcin
; The Institute of Immunology, Rockefellerova 2, 10000 Zagreb, Croatia
N. Mrkonjić
; University of Zagreb, Faculty of Chemical Engineering and Technology, Marulićev trg 19, 10000 Zagreb, Croatia
S. Kuzmić
; Forensic Science Centre “Ivan Vučetić”, Ilica 335, 10000 Zagreb, Croatia
T. Dolenec
; Sestre Milosrdnice University Hospital Center, Department of Transfusion and Regenerative Medicine, Draškovićeva 19, Zagreb, Croatia
I. Vrgoč Zimić
; Sestre Milosrdnice University Hospital Center, Department of Transfusion and Regenerative Medicine, Draškovićeva 19, Zagreb, Croatia
B. Mijović
; University of Zagreb, Faculty of Textile Technology, Prilaz baruna Filipovića 28a, 10000 Zagreb, Croatia
Sažetak
Natural polymer-based scaffolds are generally considered as favourable matrices for the adhesion and growth of cells in tissue repair. One of the most popular materials in
this respect is silk fibroin, known for its wide usage in biomedical applications. This work focuses on the development of electrospun scaffolds based on poly(ε-caprolactone) (PCL) and silk fibroin (SF) evaluated regarding the SF effect on their morphology, surface wetting ability, thermal properties, and HaCaT model cell line biocompatibility. The study revealed that the lowest PCL/SF concentration resulted in highest bead-like morphology formation, relatively thick fibers with the presence of random beads in the case of PCL, while uniform and thinner fibers in the case of increasing PCL/SF content scaffolds. The addition of SF reduced the degree of crystallinity in the PCL due to the less organized crystal structure, and decreased its thermal stability. Both SEM and MTT analyses showed cell presence on all scaffolds three days after cell seeding. Although SF improved PCL hydrophilicity, as shown quantitatively by the MTT assay for improved cytocompatibility properties, more structured electrospun PCL/SF scaffold strategies are required.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
electrospinning; poly (ε-caprolactone); silk fibroin; scaffold; HaCaT cell line
Hrčak ID:
256420
URI
Datum izdavanja:
25.4.2021.
Posjeta: 954 *