Croatica Chemica Acta, Vol. 93 No. 3, 2020.
Izvorni znanstveni članak
https://doi.org/10.5562/cca3781
Enantioseparation of (±)-trans-β-lactam Ureas by Supercritical Fluid Chromatography
Mladenka Jurin
; Department of Materials Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
Darko Kontrec
; Department of Organic Chemistry and Biochemistry Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
Tonko Dražić
; Medicinal Chemistry, Institute of Pharmacy and Molecular Biotechnology IPMB, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany
Marin Roje
orcid.org/0000-0002-7478-1490
; Department of Organic Chemistry and Biochemistry Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
Sažetak
In this study the enantioseparation of (±)-trans-β-lactam ureas 1a–g by supercritical fluid chromatography (SFC) was examined using different polysaccharide based chiral stationary phases (CSPs), and CO2/alcohol (70:30, V/V) as the mobile phase. The influence of CSP type (coated or immobilized), modifiers (alcohols), additive (isopropylamine), temperature and backpressure on enantioseparation were examined. From five tested columns, only the column filled with tris-(4-methylphenylcarbamoyl) cellulose selector proved superior in terms of broad range substrate acceptability and selectivity.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
β-lactam ureas; enantioseparation; SFC chromatography; chiral stationary phases (CSP's)
Hrčak ID:
257454
URI
Datum izdavanja:
7.3.2021.
Posjeta: 979 *