Izvorni znanstveni članak

https://doi.org/10.46419/vs.53.1.8

Infertility in dairy cows – Possible bacterial and viral causes

Attila Dobos orcid.org/0000-0003-2282-955X ; CEVA-Phylaxia Co. Ltd., Szállás u. 5, H-1107 Budapest, Hungary
István Fodor ; CEVA-Phylaxia Co. Ltd., Szállás u. 5, H-1107 Budapest, Hungary
Zsuzsa Kreizinger ; Institute for Veterinary Medical Research, Budapest, Hungary
László Makrai ; Department of Microbiology and Infectious Diseases, University of Veterinary Science, Budapest, Hungary
Béla Dénes ; National Food Chain Safety Office, Veterinary Diagnostic Directorate, Budapest, Hungary
István Kiss ; CEVA-Phylaxia Co. Ltd., Szállás u. 5, H-1107 Budapest, Hungary
Dražen Đuričić ; Mount Trade d.o.o., Garešnica, Croatia
Mislav Kovačić ; Mount Trade d.o.o., Garešnica, Croatia
Levente Szeredi ; National Food Chain Safety Office, Veterinary Diagnostic Directorate, Budapest, Hungary

Sažetak

In this research uterine swab and biopsy samples were collected from 40 infertile dairy cows kept at five dairy cattle farms in Hungary. Samples were tested for bacteria including Coxiella burnetii chlamydiae, Mycoplasma and Ureaplasma, and for the viruses Bovine herpesvirus 1 (BoHV-1) and Bovine viral diarrhoea virus (BVDV). Chlamydiaceae DNA was detected by real-time PCR in 22/40 (55%) samples. Coxiella burnetii DNA was detected in 3/40 (7.5%) cases by real-time PCR. Mycoplasma and Ureaplasma DNA was found in 2/40 (5%) and 4/40 (10%) cows, respectively. BVD and BoHV-1 DNA was not detected in any samples. Escherichia coli as a recognised uterine pathogen was found in two cases. The following potential uterine pathogens were found: Bacillus licheniformis (one case), non- haemolytic streptococci (five cases), Histophilus somni (two cases) and Candida krusei (two cases). Blood samples were collected at same time as swab samples from all 40 cows, and their examination for C. burnetii antibodies by ELISA revealed seropositivity in 26/40 cows (65%). Histological examination of the uterine biopsy samples showed the presence of mild lympho-histiocytic infiltration in the mucosain 22 cases (59%). Moderatelympho-histiocytic infiltration of the endometrium was evident in 13 cases (35%), while in two cases (6%) severe inflammatory cell infiltration of the endometrium with lympho-histiocytes and neutrophil granulocytes was found. Although no statistical correlation could be demonstrated between the severity of histological lesions of the endometrium and the uterine pathogenicity of the bacteria (P = 0.8555), endometritis of a certain severity grade and/or a recognised or potential uterine pathogen were found in all samples. The latter may play a role in the development of infertility either collectively or independently.

Ključne riječi

Bovine infertility; Dairy cow; Histological examination; Uterine biopsy; Uterine swab

Hrčak ID:

260983

URI

https://hrcak.srce.hr/260983

Datum izdavanja:

17.6.2021.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.614 *