Acta Pharmaceutica, Vol. 72 No. 2, 2022.
Izvorni znanstveni članak
https://doi.org/10.2478/acph-2022-0015
A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urine
BASMA M. SELIM
; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Sinai University, North Sinai, Al’Arish, Egypt
RANDA A. ABDELSALAM
; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
ALAA EL-GINDY
; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
BASMA G. EID
; Department of Pharmacology & Toxicology, Faculty of Pharmacy, King Abdulaziz University, Saudi Arabia
THIKRYAT NEAMATALLAH
; Department of Pharmacology & Toxicology, Faculty of Pharmacy, King Abdulaziz University, Saudi Arabia
AHDAB N. KHAYYAT
; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Saudi Arabia
GHADA M. HADAD
; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
MAHMOUD M. ELKHOUDARY
; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Horus University-Egypt, New Damietta, Egypt
Sažetak
The novelty of this work is the simultaneous analysis of sulbactam (SUL), ampicillin (AMP), and paracetamol (PARA) in human urine samples, using the environmentally benign RP-HPLC method. A C18 column was used in chromatographic separation using potassium dihydrogen phosphate (10 mmol L–1, pH 5)/ethanol (90 %, V/V) as the mobile phase; flow rate was 1.00 mL min–1. UV detection at 220 nm was used for quantification. The proposed method showed good linearity in the concentration ranges of 2.20–250.00 μg mL–1 for SUL, 2.50–250.00 μg mL–1 for PARA, and 14.50–250.00 μg mL–1 for AMP. Direct injection of urine samples with no prior extraction was performed. This method was found successful in moving towards greener studies of drugs’ urinary excretion, by decreasing hazardous solvent consumption and waste. Moreover, the method was applied to investigate the urinary excretion of the drugs and possible interaction between ampicillin and paracetamol.
Ključne riječi
ampicillin; paracetamol; sulbactam; HPLC; green analytical chemistry; drug-drug interaction; urinary excretion
Hrčak ID:
262125
URI
Datum izdavanja:
30.6.2022.
Posjeta: 1.126 *