Veterinarski arhiv, Vol. 78 No. 4, 2008.
Izvorni znanstveni članak
The use of the hypoosmotic swelling test and supravital staining in evaluation of sperm quality in boars
Marko Samardžija
orcid.org/0000-0003-0402-3173
; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Tomislav Dobranić
; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Suzana Krušlin
; Veterinary Station Krapina, Croatia
Marijan Cergolj
; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Martina Karadjole
; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Nikica Prvanović
; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Juraj Grizelj
; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
Semen samples from ten boars, Yorkshire and Pietrain breed, aged between two and six years were used. The sperm quality was evaluated using the following parameters: the motility (subjective), the structural supravital staining (eosine/nigrosine) and the functional integrity of sperm membrane (HOS test). The majority of HOS reactive or swelled up spermatozoa were gained in a hypoosmolar solution of 100 mOsm/kg by incubation of 30 minutes. Between middle values for HOS reactive (swollen up) spermatozoa in hypoosmolar solutions with different osmolarity, significant differences (P<0.05) were determined. Using a hypoosmotic solution with different osmolarity 100 mOsm/kg, significantly strong, positive correlations between HOS and motility (r = 0.98; P<0.05) were determined, between HOS test and supravital staining eosine/nigrosine (r = 0.99; P<0.05) and between motility and supravital staining eosine/nigrosine (r = 0.98; P<0.05). The results of our research have shown that the HOS test and supravital staining eosine/nigrosine could be used for routine evaluation of the functional and structural integrity of diluted boar sperm.
Ključne riječi
boar; semen; hypoosmotic test; eosine/nigrosine staining
Hrčak ID:
26535
URI
Datum izdavanja:
20.8.2008.
Posjeta: 2.558 *