Kratko priopćenje

Effect of radioprotective and chemoprotective drug (WR-2721) on toxicity of acetaminophen in mice

Joško Aleksić ; Opća bolnica Knin, Knin, Hrvatska
Domagoj Vergles ; Klinička bolnica Dubrava, Zagreb, Hrvatska
Filip Čulo ; Medicinski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska

Sažetak

Background and Purpose: It is known that WR-2721 WR-2721 – [S-2-(3-aminopropylamino)ethylposphorothioate] has radioprotective and chemoprotective effects on non-tumor tissues, and is now introduced into clinical tumor therapy protocols.We investigated whetherWR-2721 have a protective role in acute liver injury induced with acetaminophen (APAP).

Materials and Methods: CBA/H Zgr inbred mice of both sexes aged 12–16 weeks, weighing 20–25 g were used. Mice were given phenobarbitone- sodium in drinking water during 7 days (300 mg/kg) in order to induce hepatic drug-metabolizing enzymes. Thereafter, mice were fasted overnight andWR-2721 was given i.p. (50, 100 or 200 mg/kg). After 15–30 minutes they received acetaminophen (APAP; Paracetamol) 200 mg/kg by gastric tube. Animals were allowed food 4 hours later. The mortality of mice was followed for 3 days and serum aminotransferase levels were determined 24 hours after APAP administration.

Results: Survival of mice was prolonged after all doses ofWR-2721, but significantly only after the dose of 100 mg/kg of WR-2721. Similarly, the pretreatment of mice with 100 mg/kg of WR-2721 highly significantly reduced serum aspartate aminotransferase levels (AST, p<0. 0005) and serum alanine aminotransferase levels (ALT, p<0.005). The doses of 50 and 200 mg/kg of WR-2721 also reduced AST and ALT, but not significantly.

Conclusions: These data showed that WR-2721 has definitive hepatoprotective effect which is significant in very restricted dose range.

Ključne riječi

Hrčak ID:

29288

URI

https://hrcak.srce.hr/29288

Datum izdavanja:

29.2.2008.

Posjeta: 1.541 *