Izvorni znanstveni članak
Measurement of the intersystem crossing yield of s4U within tRNA by time-resolved absorption bleaching
Z. Shaqiri
; Institute of Solid State Physics, Bulgarian Academy of Sciences, 72, Tsarigradsko Shaussee blvd., 1784 Sofia, Bulgaria
D. Angelov
; Institute of Solid State Physics, Bulgarian Academy of Sciences, 72, Tsarigradsko Shaussee blvd., 1784 Sofia, Bulgaria
E. Vidolova-Angelova
; Institute of Solid State Physics, Bulgarian Academy of Sciences, 72, Tsarigradsko Shaussee blvd., 1784 Sofia, Bulgaria
Sažetak
The rare base 4-thiouridine (s4U), present in various transfer RNA (tRNA) molecules from Escherichia coli, occupies usually the strategically important 8 th position between the double helices of the acceptor and the dihydrouridine stems of the cloverleaf. This unusual base is largely used as an intrinsic build-in probe for RNA conformational and RNA(DNA)–protein interaction studies through triplet excited state photochemistry related to covalent adducts formation. Here, by applying laser transient absorption saturation spectroscopy, we measured the intersystem crossing yield ϕST and the excited triplet state absorption εT−T of s4U within tRNA. While the incorporation of s4U in tRNA induced appreciable changes in the latter, no important variation of the intersystem crossing yield was observed, which is in contrasts with the published data.
Ključne riječi
4-thiouridine (s4U); RNA conformational and RNA(DNA)–protein interaction; laser transient absorption saturation spectroscopy; intersystem crossing yield; excited triplet state absorption
Hrčak ID:
302551
URI
Datum izdavanja:
3.8.2008.
Posjeta: 459 *