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Capillary Electrophoresis Determination of Pravastatin and Separation of Its Degradation Products

Biljana Nigović ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Ivana Vegar ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia

Puni tekst: engleski, pdf (186 KB) str. 615-622 preuzimanja: 1.028* citiraj
APA 6th Edition
Nigović, B. i Vegar, I. (2008). Capillary Electrophoresis Determination of Pravastatin and Separation of Its Degradation Products. Croatica Chemica Acta, 81 (4), 615-622. Preuzeto s https://hrcak.srce.hr/31187
MLA 8th Edition
Nigović, Biljana i Ivana Vegar. "Capillary Electrophoresis Determination of Pravastatin and Separation of Its Degradation Products." Croatica Chemica Acta, vol. 81, br. 4, 2008, str. 615-622. https://hrcak.srce.hr/31187. Citirano 26.09.2020.
Chicago 17th Edition
Nigović, Biljana i Ivana Vegar. "Capillary Electrophoresis Determination of Pravastatin and Separation of Its Degradation Products." Croatica Chemica Acta 81, br. 4 (2008): 615-622. https://hrcak.srce.hr/31187
Harvard
Nigović, B., i Vegar, I. (2008). 'Capillary Electrophoresis Determination of Pravastatin and Separation of Its Degradation Products', Croatica Chemica Acta, 81(4), str. 615-622. Preuzeto s: https://hrcak.srce.hr/31187 (Datum pristupa: 26.09.2020.)
Vancouver
Nigović B, Vegar I. Capillary Electrophoresis Determination of Pravastatin and Separation of Its Degradation Products. Croatica Chemica Acta [Internet]. 2008 [pristupljeno 26.09.2020.];81(4):615-622. Dostupno na: https://hrcak.srce.hr/31187
IEEE
B. Nigović i I. Vegar, "Capillary Electrophoresis Determination of Pravastatin and Separation of Its Degradation Products", Croatica Chemica Acta, vol.81, br. 4, str. 615-622, 2008. [Online]. Dostupno na: https://hrcak.srce.hr/31187. [Citirano: 26.09.2020.]

Sažetak
A capillary zone electrophoresis method for pravastatin determination was developed and validated.
Rapid migration of negatively charged pravastatin molecule was obtained in alkaline
buffer by the application of electric field of 30 kV. Influence of the pH value and ionic strength
of running buffer, applied voltage and capillary temperature on mobility and sensitivity was
evaluated. Detection wavelength was set to 237 nm. The method was applied to the determination
of the drug in pharmaceutical dosage form. Pravastatin is a δ-hydroxy acid, which is prone
to lactonize and epimerize in a pH-dependent manner. Micellar electrokinetic chromatographic
approach was chosen to develop a method able to separate pravastatin and its degradation
products in acidic media. The proposed method allows baseline separation of hydroxy acid and
neutral lactone forms of the drug that appear as interconversion products depending on the pH
value.

Ključne riječi
pravastatin; capillary zone electrophoresis; pharmaceuticals; micellar electrokinetic chromatography; degradation products

Hrčak ID: 31187

URI
https://hrcak.srce.hr/31187

Posjeta: 1.321 *