Izvorni znanstveni članak
https://doi.org/10.5513/JCEA01/25.2.4336
The influence exerted by LEDs and fluorescent tubes, of different colors, on regenerative processes and morphogenesis of Rebutia heliosa in vitro cultures
Teodora Iuliana VIDICAN
; University of din Oradea, Faculty of Environmental Protection, Department of Agriculture, Horticulture; Oradea, code 410057, Romania
Mihai Marcel CĂRBUNAR
orcid.org/0000-0003-0609-0505
; University of din Oradea, Faculty of Environmental Protection, Department of Agriculture, Horticulture; Oradea, code 410057, Romania
*
Andra Nicoleta LAZĂR
orcid.org/0009-0006-7251-0458
; University of din Oradea, Faculty of Environmental Protection, Department of Agriculture, Horticulture; Oradea, code 410057, Romania
Ioana Maria BORZA
; University of din Oradea, Faculty of Environmental Protection, Department of Agriculture, Horticulture; Oradea, code 410057, Romania
Gabriela Argentina POPOVICIU
; University of din Oradea, Faculty of Environmental Protection, Department of Agriculture, Horticulture; Oradea, code 410057, Romania
Andrada Iulia IENCIU
; University of din Oradea, Faculty of Environmental Protection, Department of Agriculture, Horticulture; Oradea, code 410057, Romania
Mihaela Lavinia CĂRBUNAR
; University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, code 400372, Romania
Oana Maria VIDICAN
; University of din Oradea, Faculty of Environmental Protection, Department of Agriculture, Horticulture; Oradea, code 410057, Romania
* Dopisni autor.
Sažetak
The paper traces how the light source and light color influence the regenerative processes and morphogenesis in vitro cultures of Rebutia heliosa. To establish the vitroculture of R. heliosa, some explants from young plants, either buds or rounds cut from young stems and cross-sectioned were considered. The culture medium consisted of: macroelements and Fe EDTA Murashige-Skoog (1962), microelements Heller (1953), a mineral mixture to which the following vitamins were added: pyridoxine HCl, thiamine HCl and nicotinic acid (each 1 mg/l, each), m-inositol – 100 mg/l, sucrose – 20 g/l and agar-agar 7 g/l, without growth phytoregulators.The variable element was the lighting source. Fluorescent tubes and LEDs (monochrome but differently coloured) have been used: blue (λ = 470 nm), yellow (λ = 580 nm), green (λ = 540 nm), red (λ = 670 nm) as well as LED`s emitting white light (λ = 510 nm) at a luminous intensity of 1000 lux. The evolution of R. heliosa vitro cultures was monitored for 90 days and analyzed their reaction variability. Based on the comparative study, the following can be stated: the light of fluorescent tubes is more suitable for the morphogenesis of R. heliosa vitroplants, but the processes of regeneration and organogenesis are directly influenced by the source and color of light influenced, thus rhizogenesis and caulogenesis is favored by the presence of green and red light emitted by LEDs, while the white and yellow light of fluorescent tubes favors the caulogenesis and callusogenesis processes.
Ključne riječi
callusogenesis processes; fluorescent tubes; LEDs; roots; young stem
Hrčak ID:
318556
URI
Datum izdavanja:
28.6.2024.
Posjeta: 367 *