Izvorni znanstveni članak
https://doi.org/https://doi.org/10.5513/JCEA01/25.4.4337
Triticum dicoccum as a source of nutritionally valuable proteins and essential microelements for human nutrition
Dana URMINSKÁ
; Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Institute of Biotechnology, Nitra, Trieda Andreja Hlinku 2, Slovakia
*
Tatiana BOJŇANSKÁ
; Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Institute of Food Sciences, Nitra, Trieda Andreja Hlinku 2, Slovakia
Milan CHŇAPEK
; Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Institute of Biotechnology, Nitra, Trieda Andreja Hlinku 2, Slovakia
Jana URMINSKÁ
; Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Institute of Food Sciences, Nitra, Trieda Andreja Hlinku 2, Slovakia
Alena VOLLMANNOVÁ
; Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Institute of Food Sciences, Nitra, Trieda Andreja Hlinku 2, Slovakia
Pavol HAUPTVOGEL
; Research Institute of Plant Production Piešťany, National Agricultural and Food Centre, Piešťany, Bratislavská cesta 122, Slovakia
* Dopisni autor.
Sažetak
This study aimed to analyze the protein complex and the content of selected microelements in the grain of seven Triticum dicoccum (Schrank ex. Schübl) genotypes. Discontinuous fractionation of the grain protein complex shows that gliadins (60.69%) were the most predominant fraction. Together with glutenins, they represent storage proteins with a share of 82.30%. Individual genotypes differed significantly in the amount of cytoplasmic proteins represented by albumins and globulins. SDS-PAGE results show that the majority of proteins were fractions with a relative molecular weight of 33 kDa – 96 kDa. Using ELISA with G12 monoclonal antibody high content of celiac active proteins was recorded, specifically 58.63 – 83.45 mg/kg of grain. Triticum dicoccum is therefore not suitable for celiacs. The ICP-OES method was used to determine the content of microelements. Of the nutritionally significant microelements, Triticum dicoccum contained important K, Mg, Ca, Zn, Fe and Mn concentrations.
Ključne riječi
Triticum dicoccum; protein fractions; celiac active proteins; microelements
Hrčak ID:
324991
URI
Datum izdavanja:
23.12.2024.
Posjeta: 0 *