Izvorni znanstveni članak

https://doi.org/10.46419/vs.56.5.8

The impact of in ovo administration of folic acid on gga-mir-let-7k-5p, Cyclin D1(CCND1) and nerve growth factor (NGF) expression inthe cerebral cortex of chicken embryos

Tara Khoshkenar ; Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
Farhad Mashayekhi orcid.org/0000-0002-4226-2426 ; Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran *
Mahshad Mottaghi ; Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
Zivar Salehi ; Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

* Dopisni autor.

Sažetak

Folate is essential for the proper development of the brain. The gene CCND1 is responsible for the synthesis of the cyclin D1protein, which facilitates the differentiation and proliferation of neurons. Nerve growth factor (NGF) is critical for the survival and maturation of neurons. MicroRNAs are involved in regulating various phases of neurogenesis, including the proliferation of neural stem cells and differentiation of neurons. Given the important role of folic acid (FA) inthe regulation of gene expression, the aim of this study was to evaluate the effects of in ovo injection of FA on expression levels of gga-mir-let-7k-5p, CCND1, and NGF in the cerebral cortex of chicken embryos. This study involved a total of 120 hatching eggs.Of these, 40 eggs were administered FA injections into the amniotic fluid at a dosage of 150µg per egg, another 40 eggs received Phosphate buffered saline (PBS) injections (Sham)on embryonic day 11, while the remaining 40 eggs served as controls without any injections. Cortical tissues were subsequently collected on embryonic day 19, and Real-timePCR was employed to analyse the expression of mir-let-7k-5p, CCND1, and NGF. The results revealed that the expression levels of gga-mir-let-7k-5p in the FA-treated, Sham, and control groups were 0.46±0.09, 0.73±0.18,and 0.76±0.17-fold change, respectively. Additionally, the expression levels of CCND1were recorded at 1.77±0.38, 1.18±0.25, and1.11±0.28-fold change, respectively, whileNGF levels exhibited similar patterns at1.92±0.50, 1.12±0.25, and 1.04±0.21-fold change, respectively (P<0.05). Statistical analysis indicated a significant increase in the expression of CCND1 and NGF, along side a reduction in gga-mir-let-7k-5p expression in cortices treated with FA compared to the control. This suggests that the in ovo administration of FA enhances the expression of CCND1 and NGF, while decreasing gga-mir-let-7k-5p expression in the developing cerebral cortex of chicks. Enhancing our understanding of how folate impacts the activation of gene sand microRNAs related to the development of the cerebral cortex, and its potential connection to neural tube defects, will improve our knowledge of brain development, and could result in new treatment approaches to further reduce the occurrence of these abnormalities.

Ključne riječi

folate; miRNA; gene expression; brain; chick embryo

Hrčak ID:

326189

URI

https://hrcak.srce.hr/326189

Datum izdavanja:

3.4.2025.

Podaci na drugim jezicima: hrvatski

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