Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2023.64.8 4
Infection with human cytomegalovirus, EpsteinBarr virus, and high-risk types 16 and 18 of human papillomavirus in EGFR - mutated lung adenocarcinoma
Suzana Harabajsa
; Department for Pathology and Cytology, Division of Pulmonary Cytology Jordanovac, University Hospital Center Zagreb, Zagreb, Croatia
Hajdi Šefčić
; Department for Biology, Division of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Marija Klasić
; Department for Biology, Division of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Marija Milavić
; Institute of Pathology, School of Medicine, University of Zagreb, Zagreb, Croatia
Snježana Židovec Lepej
; Department of Immunology and Molecular Diagnostics, University Hospital for Infectious Diseases Zagreb, Zagreb, Croatia
Ivana Grgić
; Department of Immunology and Molecular Diagnostics, University Hospital for Infectious Diseases Zagreb, Zagreb, Croatia
Matea Zajc Petranović
; Institute for Anthropological Research, Zagreb, Croatia
Silvana Smojver-Ježek
; School of Medicine, University of Zagreb, Zagreb, Croatia
Petra Korać
orcid.org/0000-0003-4783-2114
; Department for Biology, Division of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
*
Marko Jakopović
; Department for Lung Diseases Jordanovac, University Hospital Center Zagreb, Zagreb, Croatia
* Dopisni autor.
Sažetak
Aim To assess the frequency of human cytomegalovirus
(HCMV), Epstein-Barr virus (EBV), and high-risk types of hu
-
man papillomavirus (HPV16 and HPV18) infections in lung
adenocarcinoma samples.
Methods Lung adenocarcinoma cytological smears and
their DNA isolates were obtained from patients hospital
-
ized at the Department for Lung Diseases Jordanovac, Za
-
greb, in 2016 and 2017. Overall, 67 lung adenocarcinoma
samples were examined: 34 with epidermal growth factor
receptor gene (EGFR) mutations and 33 without EGFR mu
-
tations. The EGFR mutation status and virus presence were
assessed with a polymerase chain reaction, and random
samples were additionally tested for EBV with Sanger se
-
quencing. HCMV, EBV, HPV16, and HPV18 infections were
evaluated in relation to EGFR mutation, smoking status,
and sex. A meta-analysis of available data about HPV infec
-
tion in non-small cell lung cancer was performed.
Results More frequent HCMV, EBV, HPV16, and HPV18 in
-
fections were observed in lung adenocarcinoma samples
with EGFR mutations than in samples without these muta
-
tions. Coinfection of the investigated viruses was observed
only in lung adenocarcinoma samples with mutated EGFR.
In the group with EGFR mutations, smoking was signifi
-
cantly associated with HPV16 infection. The meta-analysis
showed that non-small cell lung cancer patients with EGFR
mutations had a higher odds of HPV infection.
Conclusion HCMV, EBV, and high-risk HPV infections are
more frequent in EGFR-mutated lung adenocarcinomas,
which indicates a possible viral impact on the etiology of
this lung cancer subtype.
Ključne riječi
Hrčak ID:
331517
URI
Datum izdavanja:
31.5.2023.
Posjeta: 259 *