Izvorni znanstveni članak
https://doi.org/10.15644/asc59/2/2
In Vitro Cytotoxicity Assessment of Different Thermoplastic Aligner Materials
Ružica Bandić
; Sveučilište u Splitu Medicinski fakultet, Odjel za istraživanje u biomedicini i zdravstvu, Split, Hrvatska; Sveučilište u Splitu Medicinski fakultet, Katedra za ortodonciju, Split, Hrvatska
Ivna Vuković Kekez
; Sveučilište u Splitu Medicinski fakultet, Katedra za ortodonciju, Split, Hrvatska; Odjel za maksilofacijalnu kirurgiju KBC Split Križine, Split, Hrvatska
Vedrana Čikeš Čulić
; Sveučilište u Splitu Medicinski fakultet, Katedra za medicinsku kemiju i biokemiju, Split, Hrvatska
Ivan Galić
; Odjel za maksilofacijalnu kirurgiju KBC Split Križine, Split, Hrvatska; Sveučilište u Splitu Medicinski fakultet, Katedra za oralnu kirurgiju, Split, Hrvatska
Gordana Paić Karega
; Sveučilište u Splitu Medicinski fakultet, Katedra za ortodonciju, Split, Hrvatska
Danijela Kalibović Govorko
orcid.org/0000-0002-2598-9009
; Sveučilište u Splitu Medicinski fakultet, Katedra za ortodonciju, Split, Hrvatska; Odjel za maksilofacijalnu kirurgiju KBC Split Križine, Split, Hrvatska
*
* Dopisni autor.
Sažetak
Objectives: This study evaluated the cytotoxicity of different thermoplastic materials and the impact of thermoforming on their cytotoxicity. Materials and methods: Four thermoplastic materials were tested before and after thermoforming: Zendura A (Bay Materials), Zendura FLX (Bay Materials), Erkoloc-pro (Erkodent), and CA Pro + (Scheu Dental). The samples were stored in artificial saliva and incubated at 37 °C for 14 days to simulate conditions in the oral cavity during one stage of clear aligner treatment. Then, the saliva was diluted with a complete culture medium at three concentrations: c1=10%, c2=20% and c3=30%. Cytotoxic activities of the materials were evaluated after incubation for 4h, 24h, 48h, and 72h by using the CCK-8 assay on human oral fibroblast cells (Innoprot, REF: P10868). Results: The mean measured values of metabolically active cells below 70% of the control were for c3 of non-thermoformed Zendura A and Erkoloc-pro, and thermoformed Zendura A, after 72-hour incubation. The analysis showed differences between non-thermoformed: Zendura A and Erkoloc-pro and Zendura FLX and CA Pro +, thermoformed: Zendura A and CA Pro +, and Zendura FLX and CA Pro + at certain conditions. Differences between concentrations (c2 vs. c3 and c1 vs. c3) in the non-thermoformed group were found. There were no statistically significant differences in cytotoxicity between non-thermoformed and thermoformed groups. Conclusions: Cytotoxicity was observed in non-thermoformed and thermoformed materials, with significant differences between concentrations of the same material. However, the thermoforming process did not impact the cytotoxicity of clear aligner materials.
Ključne riječi
Removable Orthodontic Appliances; Dental materials; Cytotoxins; Long Term Adverse Effects Orthodontic appliances; Removable; Biocompatibility; Fibroblasts; Cell culture
Hrčak ID:
332198
URI
Datum izdavanja:
19.6.2025.
Posjeta: 1.160 *