Izvorni znanstveni članak
https://doi.org/10.2478/10004-1254-60-2009-1927
Comparison of Ethanol and Acetaldehyde Toxicity in Rat Astrocytes in Primary Culture
Lucija Šarc
; University Medical Centre, Poison Control Centre, Ljubljana, Slovenia
Metoda Lipnik-Štangelj
orcid.org/0000-0001-6076-6110
; University of Ljubljana, Faculty of Medicine, Ljubljana, Slovenia
Sažetak
This study compared the effects of toxicity of ethanol and its first metabolite acetaldehyde in rat astrocytes through cell viability and cell proliferation. The cells were treated with different concentrations of ethanol in the presence or absence of a catalase inhibitor 2-amino-1,2,4 triazole (AMT) or with different concentrations of acetaldehyde. Cell viability was assessed using the trypan blue test. Cell proliferation was assessed after 24 hours and after seven days of exposure to either ethanol or acetaldehyde.
We showed that both ethanol and acetaldehyde decreased cell viability in a dose-dependent manner. In proliferation studies, after seven days of exposure to either ethanol or acetaldehyde, we observed a significant dose-dependent decrease in cell number. The protein content study showed biphasic dose-response curves, after 24 hours and seven days of exposure to either ethanol or acetaldehyde. Co-incubation in the presence of AMT significantly reduced the inhibitory effect of ethanol on cell proliferation.
We concluded that long-term exposure of astrocytes to ethanol is more toxic than acute exposure. Acetaldehyde is a much more potent toxin than ethanol, and at least a part of ethanol toxicity is due to ethanol’s first metabolite acetaldehyde.
Ključne riječi
2-amino-1,2,4 triazole; AMT; cell proliferation; cell viability; trypan blue
Hrčak ID:
40677
URI
Datum izdavanja:
15.9.2009.
Posjeta: 2.135 *