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Persistent Accumulation of 4-Hydroxynonenal-Protein Adducts in Gastric Mucosa after Helicobacter Pylori Eradication

Andriy Cherkas ; Department of Internal Medicine No.1, Danylo Halytskyi National Medical University, Lviv, Ukraine
Olha Yelisyeyeva ; Department of Histology, Cytology, and Embryology, Danylo Halytskyi National Medical University, Lviv, Ukraine
Khrystyna Semen ; Department of Internal Medicine No.1, Danylo Halytskyi National Medical University, Lviv, Ukraine
Kamelija Žarković ; Division of Pathology, University Hospital Centre »Zagreb«, Zagreb, Croatia
Danylo Kaminskyy ; Department of Pharmaceutical Chemistry, Danylo Halytskyi National Medical University, Lviv, Ukraine
Ana Čipak Gašparović ; Division of Molecular Medicine, Institute »Ru|er Bošković«, Zagreb, Croatia
Morana Jaganjac ; Division of Molecular Medicine, Institute »Ru|er Bošković«, Zagreb, Croatia
Alexander Lutsyk ; Department of Histology, Cytology, and Embryology, Danylo Halytskyi National Medical University, Lviv, Ukraine
Georg Waeg ; Institute of Molecular Bioscience, University of Graz, Graz, Austria
Neven Žarkovic ; Division of Molecular Medicine, Institute »Ru|er Bošković«, Zagreb, Croatia


Puni tekst: engleski pdf 253 Kb

str. 815-821

preuzimanja: 544

citiraj


Sažetak

Recent studies indicate that oxidative stress caused by Helicobacter pylori and insufficient host antioxidant defense could play important role in pathogenesis of gastrointestinal ulcerations. By specific monoclonal antibodies we have detected weak presence of the major lipid peroxidation bioactive marker 4-hydroxynonenal (HNE) in healthy human gastric mucosa, which strongly increased in case of H. pylori-associated peptic ulcer. Considering physiological presence of HNE on one hand, and high prevalence of H. pylori associated disorders on the other, evaluation of oxidative stress after treatment is important. Therefore, in current study immunohistochemical accumulation and distribution of HNE-protein adducts in gastric mucosa was evaluated with 21 patients having H. pylori-associated duodenal peptic ulcer (DPU) before and one month after eradication of H. pylori. Although dramatic decrease in histological manifestations of inflammation was demonstrated after eradication of H. pylori, initially high immunopositivity for the HNE-protein adducts remained elevated in antrum and even increased in stomach corpus. The observed accumulation and redistribution to higher grades of HNE-immunopositivity in nuclei of glandular cells in gastric corpus indicate augmentation of oxidative stress after treatment and open possibilities for adjuvant antioxidant treatments to protect gastric mucosa from progressive oxidative stress after eradication of H. pylori infection.

Ključne riječi

4-hydroxynonenal, oxidative stress, Helicobacter pylori, eradication, peptic ulcer

Hrčak ID:

41824

URI

https://hrcak.srce.hr/41824

Posjeta: 1.068 *