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https://doi.org/10.2478/10004-1254-60-2009-1975

Co-occurrence of Aflatoxins, Ochratoxin A, Fumonisins, and Zearalenone in Cereals and Feed, Determined by Competitive Direct Enzyme-Linked Immunosorbent Assay and Thin-Layer Chromatography

Maja Šegvić Klarić ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Zdenka Cvetnić ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Stjepan Pepeljnjak ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Ivan Kosalec ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia

Puni tekst: engleski, pdf (163 KB) str. 427-433 preuzimanja: 768* citiraj
APA 6th Edition
Šegvić Klarić, M., Cvetnić, Z., Pepeljnjak, S. i Kosalec, I. (2009). Co-occurrence of Aflatoxins, Ochratoxin A, Fumonisins, and Zearalenone in Cereals and Feed, Determined by Competitive Direct Enzyme-Linked Immunosorbent Assay and Thin-Layer Chromatography. Arhiv za higijenu rada i toksikologiju, 60 (4), 427-433. https://doi.org/10.2478/10004-1254-60-2009-1975
MLA 8th Edition
Šegvić Klarić, Maja, et al. "Co-occurrence of Aflatoxins, Ochratoxin A, Fumonisins, and Zearalenone in Cereals and Feed, Determined by Competitive Direct Enzyme-Linked Immunosorbent Assay and Thin-Layer Chromatography." Arhiv za higijenu rada i toksikologiju, vol. 60, br. 4, 2009, str. 427-433. https://doi.org/10.2478/10004-1254-60-2009-1975. Citirano 22.09.2019.
Chicago 17th Edition
Šegvić Klarić, Maja, Zdenka Cvetnić, Stjepan Pepeljnjak i Ivan Kosalec. "Co-occurrence of Aflatoxins, Ochratoxin A, Fumonisins, and Zearalenone in Cereals and Feed, Determined by Competitive Direct Enzyme-Linked Immunosorbent Assay and Thin-Layer Chromatography." Arhiv za higijenu rada i toksikologiju 60, br. 4 (2009): 427-433. https://doi.org/10.2478/10004-1254-60-2009-1975
Harvard
Šegvić Klarić, M., et al. (2009). 'Co-occurrence of Aflatoxins, Ochratoxin A, Fumonisins, and Zearalenone in Cereals and Feed, Determined by Competitive Direct Enzyme-Linked Immunosorbent Assay and Thin-Layer Chromatography', Arhiv za higijenu rada i toksikologiju, 60(4), str. 427-433. https://doi.org/10.2478/10004-1254-60-2009-1975
Vancouver
Šegvić Klarić M, Cvetnić Z, Pepeljnjak S, Kosalec I. Co-occurrence of Aflatoxins, Ochratoxin A, Fumonisins, and Zearalenone in Cereals and Feed, Determined by Competitive Direct Enzyme-Linked Immunosorbent Assay and Thin-Layer Chromatography. Arh Hig Rada Toksikol. [Internet]. 2009 [pristupljeno 22.09.2019.];60(4):427-433. https://doi.org/10.2478/10004-1254-60-2009-1975
IEEE
M. Šegvić Klarić, Z. Cvetnić, S. Pepeljnjak i I. Kosalec, "Co-occurrence of Aflatoxins, Ochratoxin A, Fumonisins, and Zearalenone in Cereals and Feed, Determined by Competitive Direct Enzyme-Linked Immunosorbent Assay and Thin-Layer Chromatography", Arhiv za higijenu rada i toksikologiju, vol.60, br. 4, str. 427-433, 2009. [Online]. https://doi.org/10.2478/10004-1254-60-2009-1975

Sažetak
Aspergillus, Penicillium, and Fusarium species frequently contaminate crops. For this reason mycotoxins such as afl atoxins (AFs), ochratoxin A (OTA), fumonisins (FBs), and zearalenone (ZEA) are found in food and feed in a wide range of concentrations, depending on environmental and storage conditions.
Consumption of mycotoxin-contaminated food and feed has been associated with acute and chronic poisoning and carcinoma. The aim of this study was to determine the incidence and co-occurrence of AFs
(B1+B2+G1+G2), OTA, FBs (B1+B2+B3), and ZEA in 37 samples of cereals and feed randomly collected in 2007 from households of an endemic nephropathy (EN) area in Croatia. The mycotoxins were determined using the competitive direct ELISA test (CD-ELISA) in combination with thin-layer chromatography (TLC). The most frequent mycotoxin was ZEA (92 %, mean 318.3 μg kg-1), followed by FBs (27 %, 3690 μg kg-1), AFs (24.3 %, 4.6 μg kg-1), and OTA (16.2 %, 9.8 μg kg-1). Levels of AFs, ZEA, and FBs detected by CD-ELISA signifi cantly correlated with the TLC results. However, only one OTA-positive sample was confi rmed by TLC due to its high limit of detection. The levels of these mycotoxins were below the permissible limit for animal feed. Twenty-nine percent of cereals were contaminated with FBs, OTA, or ZEA in mass fractions above the permissible limit for humans. Co-occurrence of two toxins
varied between 4.2 % and 54 % and of three between 4.2 % and 7.6 %. Prolonged co-exposure to AFs, OTA, FBs, and ZEA might increase the risk of various chronic diseases.

Ključne riječi
food contamination; mycotoxin synergism; mycotoxins; natural carcinogens

Hrčak ID: 45377

URI
https://hrcak.srce.hr/45377

[hrvatski]

Posjeta: 1.521 *