Original scientific paper

Dot-blot enzyme immunoassay for the detection of bovine viral diarrhea virus antibodies

Farhid Hemmatzadeh ; Department of Microbiology, Faculty of Veterinary Science, University of Melbourne, Australia
Farhad Amini ; Department of Aquatic Animals Health and Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Dot-blot enzyme immunoassay (DB-EIA) was utilized for the detection of bovine viral diarrhea (BVD) antibodies in infected cattle. In this assay whole particles of the NADL strain of BVD virus were used as an antigen. A total of 1250 sera from SN and ELISA tested cows were tested for BVDV antibodies. HRPO conjugated protein G was used to detect bound BVDV antibodies using TMB as the substrate. Statistical analyses did not show any significant differences in the sensitivity of DB-EIA, to the ELISA and SN tests. The assay proved to be a simple, inexpensive, reliable and rapid tool for BVD serodiagnosis.

Keywords

dot-blot enzyme immunoassays; bovine viral diarrhea; serum neutralization test; ELISA

Hrčak ID:

45593

URI

https://hrcak.srce.hr/45593

Publication date:

10.7.2009.

Article data in other languages: croatian

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