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https://doi.org/10.2478/10004-1254-61-2010-1967

Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay

Vilena Kašuba ; Institut za medicinska istraživanja i medicinu rada, Zagreb
Ružica Rozgaj ; Institut za medicinska istraživanja i medicinu rada, Zagreb
Marija Gamulin ; Clinical Hospital Centre Zagreb, Zagreb
Ivančica Trošić ; Institut za medicinska istraživanja i medicinu rada, Zagreb

Puni tekst: engleski, pdf (95 KB) str. 1-8 preuzimanja: 392* citiraj
APA 6th Edition
Kašuba, V., Rozgaj, R., Gamulin, M. i Trošić, I. (2010). Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay. Arhiv za higijenu rada i toksikologiju, 61 (1), 1-8. https://doi.org/10.2478/10004-1254-61-2010-1967
MLA 8th Edition
Kašuba, Vilena, et al. "Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay." Arhiv za higijenu rada i toksikologiju, vol. 61, br. 1, 2010, str. 1-8. https://doi.org/10.2478/10004-1254-61-2010-1967. Citirano 18.06.2019.
Chicago 17th Edition
Kašuba, Vilena, Ružica Rozgaj, Marija Gamulin i Ivančica Trošić. "Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay." Arhiv za higijenu rada i toksikologiju 61, br. 1 (2010): 1-8. https://doi.org/10.2478/10004-1254-61-2010-1967
Harvard
Kašuba, V., et al. (2010). 'Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay', Arhiv za higijenu rada i toksikologiju, 61(1), str. 1-8. doi: https://doi.org/10.2478/10004-1254-61-2010-1967
Vancouver
Kašuba V, Rozgaj R, Gamulin M, Trošić I. Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay. Arh Hig Rada Toksikol. [Internet]. 2010 [pristupljeno 18.06.2019.];61(1):1-8. doi: https://doi.org/10.2478/10004-1254-61-2010-1967
IEEE
V. Kašuba, R. Rozgaj, M. Gamulin i I. Trošić, "Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay", Arhiv za higijenu rada i toksikologiju, vol.61, br. 1, str. 1-8, 2010. [Online]. doi: https://doi.org/10.2478/10004-1254-61-2010-1967

Sažetak
Irinotecan is a topoisomerase I interactive agent, widely used in the treatment of metastatic colorectal cancer. The genotoxic effects of the maximum single dose (18 μg mL-1), recommended monotherapy dose (9 μg mL-1), and recommended combined therapy dose (4.5 μg mL-1) of irinotecan were studied on V79 cells using the comet assay, chromosome aberration assay, and micronucleus test. The cells were treated with irinotecan for 2 h or 24 h. The statistical signifi cance of the results was determined using the one-way ANOVA test and a nonparametric Mann Whitney U test. The comet assay did not show dose-dependent or time-dependent effects. The chromosome aberration analysis showed large DNA rearrangements, i.e.,
chromosome exchanges. Although the exposed cultures showed a signifi cant increase in micronucleated cells in respect to control, no dose-dependent relation was established among the treated cultures. Timedependent effect was also not observed.

Ključne riječi
alkaline comet assay; antineoplastic drug; cell cultures; DNA and cytogenetic damage; topoisomerase

Hrčak ID: 49011

URI
https://hrcak.srce.hr/49011

[hrvatski]

Posjeta: 784 *