Skoči na glavni sadržaj

Izvorni znanstveni članak

Effects of Vanadate on Glucose Production in Cultured Hepatocytes Isolated from Rats on High Saturated Fat Diet

Jagoda Roša
Hana Skala
Josip Roša

Puni tekst: engleski pdf 101 Kb

str. 693-696

preuzimanja: 442



Insulin resistance is a common phenomenon in obesity and Type 2 diabetes. Common factor important for development of diabetes and insulin resistance is intake of saturated fat. Vanadate treatment improves glucose homeostasis in vivo. The aim of this study was to find out changing of hepatic glucose output in dependence of saturated fat diet and
possible direct action of vanadate in cultured hepatocytes. Hepatocytes were isolated by a collagenase perfusion technique and cultured for 24 h in M 199 serum-free medium. The glucose production in hepatocytes isolated from rats on high saturated fat diet was significantly 139% higher comparable to standard controls. Glucagon 100% increased glucose production in hepatocytes from rats on standard diet and 200% in hepatocytes on saturated high fat diet. The addition vanadate significantly decreased basic glucose production and did not influence glucagon stimulated glucose production. Presence of insulin did not influence either glucagon or vanadate effect. High saturated fat diet not only increases insulin resistance but also decreases chances of successful therapy of diabetes.

Ključne riječi

insulin; glucagon; glucose production; gluconeogenesis; vanadate

Hrčak ID:



Datum izdavanja:


Posjeta: 891 *