Izvorni znanstveni članak

Diagnostic values of acute phase proteins in Iranian indigenous cattle infected with Theileria annulata

Saeed Nazifi ; Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Seyyed M. Razavi ; Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mahdieh Reiszadeh ; Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Zahra Esmailnezhad ; Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Maryam Ansari-Lari ; Department of Food Hygiene, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Sažetak

This study was conducted to assess the pattern of changes and the relative value of acute phase proteins (APP) including haptoglobin (Hp), serum amyloid A (SAA), ceruloplasmin and fi brinogen in Iranian indigenous cattle infected with Theileria annulata. The diseased group comprised 24 Iranian indigenous dairy cattle, 2-3 years old, naturally infected with Theileria annulata. The infected animals were divided into 4 subgroups with different parasitemia rates (<1% and 1-3%). As a control group, 10 uninfected cattle were also sampled. Blood samples were collected and all measurements were made using validated methods. There were significant differences in red blood cells (RBCs), packed cell volume (PCV), hemoglobin (Hb) and concentrations of Hp, SAA, ceruloplasmin and fibrinogen between healthy cattle and those infected with T. annulata with different parasitemia rates (P<0.05). As the parasitemia rate increased in infected cattle, a signifi cant decrease was
observed in RBCs, PCV and Hb. In contrast, with the increase in the parasitemia rate, a significant increase in Hp, SAA, ceruloplasmin and fibrinogen was evident. The optimal cut-off point was set by the receiver operating characteristics (ROC) method to >5.68 μg/mL for SAA, >0.09 g/L for Hp, >0.049 g/L for ceruloplasmin and >1.90 g/L for fibrinogen, with corresponding 71.50% sensitivity and 100% specificity for SAA, 83.30% sensitivity and 70% specificity for Hp, 50% sensitivity and 90% specificity for ceruloplasmin and 71.30% sensitivity and 80% specificity for fi brinogen. In conclusion, measuring SAA with the highest sensitivity, specificity and AUC compared to other APPs, can be a suitable indicator of inflammatory reactions in indigenous cattle infected with Theileria annulata.

Ključne riječi

haptoglobin; serum amyloid A; ceruloplasmin; fibrinogen; Iranian indigenous dairy cattle; Theileria annulata

Hrčak ID:

56265

URI

https://hrcak.srce.hr/56265

Datum izdavanja:

26.4.2010.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.952 *