Original scientific paper

Characterization of the ICP4 gene in pathogenic Marek’s disease virus of poultry in Gujarat, India, using PCR and sequencing

Irshadullakhan H. Kalyani ; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Mahesh M. Tajpara ; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Mayur K. Jhala ; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Bharat B. Bhanderi ; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Jitendra B. Nayak ; Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Jayantilal H. Purohit ; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India

Abstract

A total of 34 clinical samples were collected for detection of Marek’s disease virus (MDV) by polymerase chain reaction assays using primers M1.1/M1.8 to amplify a region of the ICP4 gene in layer birds of poultry. Primer set M1.1/M1.8 amplified a 318 bp product as against the expected 247 bp product in 30 samples out of 34 samples tested. To confirm the result, this primer was subjected to NCBI BLAST, and it was found that the primer specific segment of 318 bp does exist in the published sequence of Md5 and Md11BAC. The PCR product was sequenced and resulted in 273bp by direct sequencing. The sequence was analysed using NCBI blast and Clustal W with the published sequence of Gallid herpes virus-2 giving a matching score of 97, 96 and 90% indicating a highly conserved region. This shows that the MDV is prevalent in Gujarat.

Keywords

Marek’s disease virus; ICP4 gene; polymerase chain reaction

Hrčak ID:

62162

URI

https://hrcak.srce.hr/62162

Publication date:

26.10.2010.

Article data in other languages: croatian

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