Original scientific paper

Molecular characterization and computational analysis of the major outer membrane protein (ompH) gene of Pasteurella multocida P52.

Rashmi Singh ; Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
Kamal Tewari ; Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
Nandakumar Packiriswamy ; Department of Comparative Medicine and Integrated Biology, College of Veterinary Medicine, Michigan State University, USA
Soma Marla ; Division of NRC on DNA Finger Printing, National Bureau of Plant Genetics Resources, New Delhi, India
Velagapudi D. P. Rao ; Department of Poultry and Fish Diseases, AL-Fateh University, Tripoli, Libya

Abstract

The major outer membrane protein (OmpH) of P. multocida P52 was identified as one of the major immunodominant antigens. The gene ompH, encoding OmpH, was amplified, cloned and sequenced. The coding region of OmpH is 1,002 bp long. The predicted primary protein is composed of 333 amino acids, with a 20-amino acid signal peptide. The mature protein contains 313 amino acids with a predicted molecular mass of 33,760 Da. The nucleotide sequence and the predicted amino acid sequence of the ompH gene of P. Multocida P52 showed a high level of homology to the OmpH of other serotypes of P. multocida, confirming that the ompH gene is conserved among all the serotypes of P. multocida. Multiple sequence alignment revealed high homology among the serotypes, with major variations confined to two discrete regions (amino acids 82-102 and 223-240), which corresponded to hydrophilic domains showing high antigenicity. The sequence information, presented in this study will open new vistas in progress towards the development of suitable prophylaxis and molecular epidemiological analysis.

Keywords

gene; haemorrhagic septicaemia; outer membrane protein; Pasteurella multocida; sequencing

Hrčak ID:

67327

URI

https://hrcak.srce.hr/67327

Publication date:

26.4.2011.

Article data in other languages: croatian

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