APA 6th Edition Puc, M., Humar, I. i Bulić-Jakuš, F. (2011). Lymph Node, Spleen and Peripheral Blood Lymphocytes as Stimulators of Alloreactivity. Collegium antropologicum, 35 (2), 369-376. Preuzeto s https://hrcak.srce.hr/68947
MLA 8th Edition Puc, Maja, et al. "Lymph Node, Spleen and Peripheral Blood Lymphocytes as Stimulators of Alloreactivity." Collegium antropologicum, vol. 35, br. 2, 2011, str. 369-376. https://hrcak.srce.hr/68947. Citirano 03.08.2020.
Chicago 17th Edition Puc, Maja, Ines Humar i Floriana Bulić-Jakuš. "Lymph Node, Spleen and Peripheral Blood Lymphocytes as Stimulators of Alloreactivity." Collegium antropologicum 35, br. 2 (2011): 369-376. https://hrcak.srce.hr/68947
Harvard Puc, M., Humar, I., i Bulić-Jakuš, F. (2011). 'Lymph Node, Spleen and Peripheral Blood Lymphocytes as Stimulators of Alloreactivity', Collegium antropologicum, 35(2), str. 369-376. Preuzeto s: https://hrcak.srce.hr/68947 (Datum pristupa: 03.08.2020.)
Vancouver Puc M, Humar I, Bulić-Jakuš F. Lymph Node, Spleen and Peripheral Blood Lymphocytes as Stimulators of Alloreactivity. Collegium antropologicum [Internet]. 2011 [pristupljeno 03.08.2020.];35(2):369-376. Dostupno na: https://hrcak.srce.hr/68947
IEEE M. Puc, I. Humar i F. Bulić-Jakuš, "Lymph Node, Spleen and Peripheral Blood Lymphocytes as Stimulators of Alloreactivity", Collegium antropologicum, vol.35, br. 2, str. 369-376, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/68947. [Citirano: 03.08.2020.]
Sažetak Before and after kidney transplantations, in vitro tests that measure the level of reactivity between donor and recipient
lymphocytes are performed for better organ selection and as indicator of possible organ rejection. In these tests, donor’s
and recipient’s lymphocytes are stimulated for proliferation, which intensity is measured and accordingly organ recipient
reactivity towards graft is determined. Lymph node, spleen and peripheral blood lymphocytes are used for those purposes.
For better interpretation of these in vitro tests it should be important to determine mitogenic ability of lymphocytes
of different origin and to choose the most adequate cells. To compare mitogenic ability of deceased donor lymph node,
spleen and peripheral blood lymphocytes one-way mixed lymphocyte culture (MLC) was used. As stimulators irradiated
lymphocytes from spleen, lymph node and peripheral blood samples of 12 deceased donors were used while as responders
lymphocytes from peripheral blood of healthy individuals, chosen according HLA-DRB1 alleles (stimulators and responders
were HLA-DRB1 identical, semi-identical or different), were used. Spleen lymphocyte activity was the best with
different cells and the weakest with identical cells. Impact of polyclonal mitogens (PHA – phytohemagglutinin, Con A –
concanavalin A and PWM – pokeweed mitogen) on lymphocyte proliferation was tested on lymphocytes from spleen and
lymph node of deceased donors. Results abtained in culture in vitro showed that spleen cells had exerted the best mitogenic
potential and PHA had the greatest impact upon lymphocyte proliferation. This investigation is of importance for
establishing the best model to reflect in vivo situation in transplanted patient.