Izvorni znanstveni članak

https://doi.org/10.5562/cca1765

Capillary Electromigration Techniques for the Quantitative Analysis of Colchicine

Ede Bodoki ; “Iuliu Hatieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Analytical Chemistry Department, 4 Louis Pasteur St., 400349, Cluj-Napoca, Romania
Bogdan Cezar Iacob ; “Iuliu Hatieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Analytical Chemistry Department, 4 Louis Pasteur St., 400349, Cluj-Napoca, Romania
Radu Oprean ; “Iuliu Hatieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Analytical Chemistry Department, 4 Louis Pasteur St., 400349, Cluj-Napoca, Romania

Sažetak

The separation and UV absorbance detection of colchicine by three different capillary electrophoretic
methods is described. Colchicine is known as a neutral compound, being able to be determined
by electrokinetic chromatography. For the first time, a non-aqueous capillary electrophoretic method is
described, based on the electromigration of ionized colchicine induced by 10 mM HClO4 in a mixture of
methanol:acetonitrile (1 :2, v/v) containing 60 mM ammonium formiate, opening up new perspectives in
the trace analysis of the highly toxic drug from clinical and food samples using online coupling of the CE
system to a mass spectrometer. For the quantitative assessment of colchicine content from meadow saffron
(Colchicum autumnale L.) seeds and 1 mg colchicine tablets, a simple, quick and sensitive micellar
electrokinetic chromatographic method was developed and fully validated according to ICH guidelines in
terms of selectivity, linearity, accuracy, intermediate precision and limits of detection and quantification
(95.2 ng mL–1). In order to further improve the detection limits, allowing the analysis of trace levels of
colchicine in biosamples and food products, an on-column preconcentration using sweeping-MEKC was
investigated. Linearity of response was observed on 10–160 ng mL–1 colchicine, with an estimated detection
limit of around 3 ng mL–1 colchicine. By further improving the affinity of the separation vector towards
colchicine this limit could be further decreased. Preliminary application of the method for the detection
of trace amounts of colchicine spiked in non-fat milk and human urine (10 ng mL–1) shows encouraging
results. (doi: 10.5562/cca1765)

Ključne riječi

colchicine; capillary electrophoresis; NACE; MEKC; sweeping-MEKC

Hrčak ID:

74777

URI

https://hrcak.srce.hr/74777

Datum izdavanja:

13.11.2011.

Posjeta: 2.763 *