Izvorni znanstveni članak

https://doi.org/10.5562/cca1787

Comparative Study of Captopril Derivatization Reaction by LC-UV, LC-MS and CE-UV Methods

Gabriella Donáth-Nagy ; University of Medicine and Pharmacy of Târgu-Mureş, Faculty of Pharmacy, Department of Physical Chemistry, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Szende Vancea ; University of Medicine and Pharmacy of Târgu-Mureş, Faculty of Pharmacy, Department of Physical Chemistry, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Silvia Imre ; University of Medicine and Pharmacy of Târgu-Mureş, Faculty of Pharmacy, Department of Drugs Analysis, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania

Sažetak

The LC-UV, LC-MS and CE-UV study of chemical reaction between captopril and p-bromophenacyl
bromide as derivatizing reagent is reported. During transformation of captopril, its thiol group
is involved and the reaction is irreversible. Neutral or alkaline environments favor derivatization. The
yield of reaction increases linear with the concentration of the reagent, while changes in temperature do
not influence it significantly. Kinetic studies show that derivatization of captopril flows quickly, reaching
a plateau after 30 minutes. Based on the study of these effects some important parameters such as deri- vatizing
agent concentration, reaction time, temperature and pH were confined. The results were used
for development of captopril determination methods both in pharmaceutical formulations and biological
samples. (doi: 10.5562/cca1787)

Ključne riječi

captopril; derivatization; LC-UV; LC-MS; CE-UV

Hrčak ID:

74808

URI

https://hrcak.srce.hr/74808

Datum izdavanja:

13.11.2011.

Posjeta: 2.492 *