Izvorni znanstveni članak
The Distribution of HLA Alleles among Children with Atopic Asthma in Croatia
Irena Ivković-Jureković
Renata Žunec
Vesna Balog
Zorana Grubić
Sažetak
Allergic asthma is a multifactorial disease involving well known environmental factors and less identified genetic
components. In several studies the HLA genes have been implicated in the development of asthma and atopy, but the importance
of these associations remains unclear. The aim of the present study was to analyse the distribution of specificities
at HLA class I loci (-A and -B) and HLA class II locus (-DRB1) in a group of 143 Croatian children with atopic
asthma, regarding total serum IgE and specific IgE against common inhalant allergens, as well as their connection with
different asthmatic phenotypes and to identify HLA genotype which increases the risk for atopy or asthma or which has a
protective effect. As controls we used a group of 163 healthy unrelated individuals. HLA class I antigens were determined
by serology, while DRB1 specificities were detected by polymerase-chain reaction amplification and hybridisation with
sequence specific oligonucleotide probes method (PCR-SSOP). We found no significant correlation between any of the
HLA-A antigens and asthma, atopy or associated atopic phenotypes. At HLA-B locus, HLA-B8 antigen was significantly
increased among asthmatic patients (p=0.002), patients with high total serum IgE (p=0.002), as well as among patients
sensitizated to Dermatophagoides pteronyssinus (Der p) (p=0.014) and among patients sensitizated to Der p + Dactylis
glomerata (Dact g) or Ambrosia elatior (Amb a) (p=0.004). Among HLA-DRB1 specificities, HLA-DRB1*01 showed positive
correlation with asthma and atopy (p=0.034), while HLA-DRB1*03 specificity was observed with significantly
higher frequency among patients with total serum IgE 400 KU/L (p=0.048). HLA-DRB1*16 specificity was observed
with significantly lower frequency among patients with asthma only in comparison to healthy controls (p=0.027) and to
patients with asthma and allergic rhinitis (p=0.005). In conclusion, our data suggest that HLA specificities play a relevant
role in predisposition to asthma, as well as in different clinical forms of atopic diseases. HLA-B8, HLA-DRB1*01
and HLA-DRB1*03 genotype increases the risk for atopic asthma and high serum IgE.
Ključne riječi
atopy; asthma; children; HLA class I and class II
Hrčak ID:
75648
URI
Datum izdavanja:
30.12.2011.
Posjeta: 1.333 *