Izvorni znanstveni članak
https://doi.org/10.2478/10004-1254-63-2012-2155
Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients in Iran
Ali Reza Khosravi
; Mycology Research Center, Faculty of Veterinary Medicine, Tehran, Iran
Mahnaz Fatahinia
; Mycology Research Center, Faculty of Veterinary Medicine, Tehran, Iran
Hojjatollah Shokri
; Faculty of Veterinary, Iran
Mohammad Hossein Yadegari
; Department of Mycology, Faculty of Medicine, University of Tarbiat Modares, Tehran, Iran
Sažetak
We extracted Fusarium solani antigens to evaluate specifi c anti-F. solani IgE in fifty-one patients with asthma (33 men and 18 women) and in 22 non-atopic healthy subjects (15 men and 7 women). F. solani strains were cultured in Sabouraud glucose agar and subjected to cell disruption using the freeze-and-thaw method. The obtained cytoplasmic extracts were analysed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Sensitisation to F. solani antigens has been evaluated in asthmatic patients using the immunoblotting assay. The SDS-PAGE identifi ed 29 protein bands in the cytoplasmic extracts of F. solani isolates, with molecular weights ranging from 24 kDa to 112 kDa. Immunoblotting detected specific anti-F. solani IgE antibody in all asthma patients, but not in the control group. The predominant reactive allergens in patients corresponded to the bands with molecular weights of 24 kDa, 58.5 kDa, 64.5 kDa, 69 kDa, 72 kDa, and 97 kDa. Our results suggest that various allergenic components of F. solani may produce symptoms of asthma in susceptible individuals and they call for further research.
Ključne riječi
airborne spores; allergenic sensitisation; fungal allergy; IgE; SDS-PAGE
Hrčak ID:
78630
URI
Datum izdavanja:
21.3.2012.
Posjeta: 2.120 *