Izvorni znanstveni članak
Frequency of loss of heterozygosity of the NF2 gene in schwannomas from Croatian patients
Nives Pećina-Šlaus
orcid.org/0000-0002-3334-7671
; Laboratory of Neurooncology, Croatian Institute for Brain Research, School of Medicine University of Zagreb, Zagreb, Croatia
Martina Zeljko
; Laboratory of Neurooncology, Croatian Institute for Brain Research, School of Medicine University of Zagreb, Zagreb, Croatia
Hrvoje Ivan Pećina
; Department of Radiology, Sestre Milosrdnice University Hospital, Zagreb, Croatia
Tamara Nikuševa Martić
; Department of Biology, School of Medicine, University of Zagreb,Zagreb, Croatia
Niko Bačić
; Laboratory of Neurooncology, Croatian Institute for Brain Research, School of Medicine University of Zagreb, Zagreb, Croatia
Davor Tomas
; Ljudevit Jurak Department of Pathology, Sestre Milosrdnice University Hospital, Zagreb, Croatia
Reno Hrašćan
; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Sažetak
Aim To identify gross deletions in the NF2 gene in a panel
of schwannomas from Croatian patients in order to establish
their frequencies in Croatian population.
Methods Changes of the NF2 gene were tested by polymerase
chain reaction/loss of heterozygosity (LOH) using
two microsatellite markers, D22S444 and D22S929.
Results The analysis with both markers demonstrated that
43.75% of schwannomas exhibited LOH of the NF2 gene.
The D22S444 region exhibited 45.5% of LOHs and the
D22S929 region exhibited 14.3% of LOHs. Four LOHs were
found in Antoni B, 2 in Antoni A, and 1 in Antoni A and B
type tumors.
Conclusion The frequency of changes observed in Croatian
patients is broadly similar to that reported in other
populations and thus confirms the existing hypothesis regarding
the tumorigenesis of schwannomas and contributes
to schwannoma genetic profile helping us to better
understand its etiology and treatment.
Ključne riječi
Hrčak ID:
85775
URI
Datum izdavanja:
15.8.2012.
Posjeta: 1.117 *