hrcak mascot   Srce   HID

Izvorni znanstveni članak

Differentiation of infectious bursal disease virus strains at a genomic level.

Zdenko Biđin ; Department of Poultry Pathology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivana Lojkić ; Department of Poultry Pathology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Magdalena Grce ; Division of Molecular Medicine, Institute Ruđer Bošković, Zagreb, Croatia
Stanislav Čajavec ; Veterina d.o.o., Kalinovica, Croatia
Biserka Pokrić ; Division of Molecular Medicine, Institute Ruđer Bošković, Zagreb, Croatia

Puni tekst: engleski, pdf (154 KB) str. 325-336 preuzimanja: 748* citiraj
APA 6th Edition
Biđin, Z., Lojkić, I., Grce, M., Čajavec, S. i Pokrić, B. (2001). Differentiation of infectious bursal disease virus strains at a genomic level.. Veterinarski arhiv, 71 (6), 325-336. Preuzeto s https://hrcak.srce.hr/90126
MLA 8th Edition
Biđin, Zdenko, et al. "Differentiation of infectious bursal disease virus strains at a genomic level.." Veterinarski arhiv, vol. 71, br. 6, 2001, str. 325-336. https://hrcak.srce.hr/90126. Citirano 29.11.2021.
Chicago 17th Edition
Biđin, Zdenko, Ivana Lojkić, Magdalena Grce, Stanislav Čajavec i Biserka Pokrić. "Differentiation of infectious bursal disease virus strains at a genomic level.." Veterinarski arhiv 71, br. 6 (2001): 325-336. https://hrcak.srce.hr/90126
Harvard
Biđin, Z., et al. (2001). 'Differentiation of infectious bursal disease virus strains at a genomic level.', Veterinarski arhiv, 71(6), str. 325-336. Preuzeto s: https://hrcak.srce.hr/90126 (Datum pristupa: 29.11.2021.)
Vancouver
Biđin Z, Lojkić I, Grce M, Čajavec S, Pokrić B. Differentiation of infectious bursal disease virus strains at a genomic level.. Veterinarski arhiv [Internet]. 2001 [pristupljeno 29.11.2021.];71(6):325-336. Dostupno na: https://hrcak.srce.hr/90126
IEEE
Z. Biđin, I. Lojkić, M. Grce, S. Čajavec i B. Pokrić, "Differentiation of infectious bursal disease virus strains at a genomic level.", Veterinarski arhiv, vol.71, br. 6, str. 325-336, 2001. [Online]. Dostupno na: https://hrcak.srce.hr/90126. [Citirano: 29.11.2021.]

Sažetak
Reverse transcription-polymerase chain reaction (RT-PCR) followed by restriction fragment-length polymorphism (RFLP) analysis was tested in order to determine its accuracy and sensitivity for differentiation of infectious bursal disease virus (IBDV) strains. Two commercial vaccine strains and two Croatian field isolates were analysed. RT-PCR assay was used for amplification of a variable part of VP2 gene (422 bp) encoding for major neutralizing epitopes. Amplified VP2 fragments were further characterized by RFLP analysis using the SacI and TaqI restriction endonucleases. Both field isolates showed RFLP pattern similar to highly virulent European and Asian strains. Both vaccine strains had RFLP profiles different from that of the field isolates. Vaccinal strain 2512 had RFLP pattern similar to mild vaccinal strain pbg-98, and strain VMG-91 was similar to other classical vaccinal strains of intermediate pathogenicity.

Ključne riječi
infectious bursal disease; RT-PCR; RFLP; vaccinal strains; field isolates

Hrčak ID: 90126

URI
https://hrcak.srce.hr/90126

[hrvatski]

Posjeta: 1.021 *