Izvorni znanstveni članak
The T945M Single Nucleotide Polymorphism of the Bovine Leptin Receptor Gene in Population of Slovak Spotted Bulls
Anna Trakovická
; Slovak University of Agriculture in Nitra, Department of Animal Genetics and Breeding Biology, Tr. A. Hlinku 2, 949 76 Nitra, The Slovak Republic
Nina Moravčíková
orcid.org/0000-0003-1898-8718
; Slovak University of Agriculture in Nitra, Department of Animal Genetics and Breeding Biology, Tr. A. Hlinku 2, 949 76 Nitra, The Slovak Republic
Martina Miluchová
; Slovak University of Agriculture in Nitra, Department of Animal Genetics and Breeding Biology, Tr. A. Hlinku 2, 949 76 Nitra, The Slovak Republic
Sažetak
The objective of this study was detection of DNA polymorphism of the leptin receptor gene using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The SNP T945M, which maps on bovine chromosome 3 at the exon 20 of the leptin receptor sequence and corresponds to a mutation in the intracellular region of the functional protein, was also analyzed. In exon 20, a T to C missense mutation was found at nucleotide 115, which causes an amino acid substitution at residue 945 (T954M). The polymorphism of leptin receptor gene was studied in a group of 57 bulls of Slovak spotted breed. A strategy employing PCR was used to amplify 197 bp products from blood samples. Digestion of PCR products with restriction enzyme BseGI revealed two alleles: allele C was 130 and 67 fragments and allele T was 93, 67 and 37. Frequencies for allele C and T were 0.9737 and 0.0263, respectively and TT genotype was not detected.
Ključne riječi
bovine leptin receptor gene; cattle; polymorphism; SNP T945M
Hrčak ID:
72026
URI
Datum izdavanja:
3.10.2011.
Posjeta: 813 *