Veterinarski arhiv, Vol. 73 No. 6, 2003.
Izvorni znanstveni članak
Immunophenotyping of cells involved in early immune response to experimental Trichinella spiralis gut infection.
Frane Božić
; 1Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Albert Marinculić
; Department of Parasitology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Relja Beck
; Department of Parasitology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
Peyer's patches (PP) are believed to be the principal sites for the induction of immune responses to invading mucosal pathogens, yet the phenotype of T cells recruited within the PP during the early phase of Trichinella spiralis gut infection is largely unexplored. In order to gain an insight into this issue, in the present study C57BL mice were orally infected with the parasite at day 0 and necropsied at 1, 4, 7 and 14 days post infection (p. i.). Uninfected mice served as control. Absolute number of mononuclear PP cells and cell surface marker expression of PP T cell subpopulations in the infected mice are compared with those from spleen. Our results showed that early during the intestinal phase of infection, total number of PP mononuclear cells gradually declined from p. i. day 1 onward, whereas the number of splenocytes dramatically increased at 4 and 14 days p. i. At the same time, no major changes in the phenotype of PP T cells were observed in infected versus uninfected mice. However, lymphocytes from spleen underwent significant kinetic changes related to the infection.
Ključne riječi
Trichinella spiralis; immunophenotyping; T cells; flow cytometry
Hrčak ID:
76476
URI
Datum izdavanja:
21.12.2003.
Posjeta: 1.135 *