Izvorni znanstveni članak

Age-related changes in porcine humoral and cellular immune parameters.

Dalibor Potočnjak ; Clinic of Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Dubravko Kezić ; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Maja Popović ; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Nevijo Zdolec ; Department of Hygiene and Technology of Animal Foodstuffs, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Hrvoje Valpotić ; Department of Animal Nutrition, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia;Division of Biology
Vesna Benković ; Department of Animal Physiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Gordan Mršić ; Forensic Science Centre “Ivan Vučetić”, Zagreb, Croatia
Ana Kovšca Janjatović ; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Gordana Lacković ; Department of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Ivica Valpotić ; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Sažetak

The sequence of development of porcine systemic and local humoral and cellular immunity was analyzed by the age-dependent patterns of total serum immunoglobulin (Ig) levels, responsiveness of peripheral blood lymphocytes (PBL) to common mitogens (phytohemagglutinin, PHA; concanavalin A, ConA; poke-weed mitogen, PWM), or allogeneic PBL in a mixed lymphocyte culture (MLC), and the expression/distribution patterns of intestinal mucosal immune cell subsets, in order to establish the basic immune parameters for an assessment of immunocompetence in clinically normal pigs. Thus, we surveyed the species-related physiological values of these parameters in different age/technological categories, comprising infant (suckling and weaned pigs) and adult (gilts, sows, and boars) swine, kept in intensive rearing conditions. The highest level of total Igs was determined in neonatal pigs (43.5 ± 4.6 g/L), and this value was significantly higher (P<0.001) than that in weaned pigs (12.1 ± 1.5 g/L). Among adult swine, the highest level of total Igs was recorded in sows (32.0 ± 3.1 g/L), and this was much higher (P<0.001) than that found in gilts (25.1 ± 3.5 g/L) or boars (21.2 ± 5.0 g/L). The reactivity of PBL in neonatal pigs decreased by 60-70% compared to weaned pigs, regardless ofthe stimulator applied. In adult swine, the strongest reactivity of PBL, except to PHA, was determined in sows. Histomorphometric analyses showed that CD45RA+, CD45RC+ and IgA+ cells in sections of the jejunum and ileum were significantly higher in weaned pigs (P<0.05) compared to neonatal pigs. The immune parameters obtained for a sample of the swine population in Croatia may serve as a basis for further research on porcine systemic and local (intestinal) immune responsiveness regarding (i) differences among breeds, (ii) the influence of paragenetic factors, (iii) the impact of microbial etiology diseases, particularly those accompanied by immunosuppression, and (iv)the validation of specific/nonspecific immunomodulation.

Ključne riječi

age-dependent; total immunoglobulin levels; lymphocyte reactivity; swine

Hrčak ID:

78256

URI

https://hrcak.srce.hr/78256

Datum izdavanja:

8.3.2012.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.074 *