Veterinarski arhiv, Vol. 72 No. 4, 2002.
Izvorni znanstveni članak
Effect of some inhibitors on neuraminidase of Newcastle disease virus Kudu 113 strain.
Sunday Blessing Oladele
; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
Paul Abdu
; Department of Surgery and Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
Andrew Jonathan Nok
; Department of Biochemistry, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria
King Akpofure Nelson Esievo
; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
Nicodemus Maashin Useh
; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
Sažetak
A total of two hundred samples from dialysed neuraminidase of Newcastle disease virus (NDV) Kudu 113 strain were used for the experiment. Fifty samples each were used for various concentrations of paranitrophenyl oxamic acid (PNPO), salicyl oxamic acid (SOA), silver nitrate (AgNO3) and ethylene diamine tetra acetic acid (EDTA), to test their inhibition effects on the neuraminidase activity of NDV Kudu 113 strain in vitro, using thiobarbituric acid assay method. Of these inhibitors, AgNO3 had the highest inhibition effect on the neuraminidase activity of NDV Kudu 113 strain. Silver nitrate reduced the activity of this enzyme from 140.24 μMol/min for enzyme without AgNO3 to 32.36 μMol/min when AgNO3 was added to the enzyme at a concentration of 0.47 M. Paranitrophenyl oxamic acid had the least inhibition effect on the neuraminidase activity of NDV Kudu 113 strain at a concentration of 0.12 M. This inhibitor reduced the activity of the enzyme from 97.08 μMol/min for enzyme without PNPO to 91.70 μMol/min when PNPO was added to the enzyme at a concentration of 0.12 M. Ethylene diamine tetra acetic acid and SOA also greatly inhibited the activity of this enzyme. Salicyl oxamic acid reduced the activity of NDV Kudu 113 strain neuraminidase from 140.24 μMol/min for enzyme without SOA to 59.33 μMol/min when SOA was added at a concentration of 0.47 M. Similarly, EDTA reduced the activity of this enzyme from 124.06 μMol/min forenzyme without EDTA to 75.51 μMol/min when EDTA was added to the enzyme at a concentration of 0.35 M. In all the inhibitors examined, it was observed that their effects on the activity of NDV Kudu 113 strain neuraminidase increased as the concentrations of the inhibitors increased. It was concluded that PNPO, SOA, EDTA and AgNO3 inhibited the neuraminidase activity of NDV Kudu 113 strain in vitro. Therefore, further studies are needed to see if these inhibitors could be of use, after proper clinical trials, as potential agents for reducing Newcastle disease (ND) infections in poultry.
Ključne riječi
inhibitor; neuraminidase; Newcastle disease virus
Hrčak ID:
80026
URI
Datum izdavanja:
17.8.2002.
Posjeta: 914 *