Veterinarski arhiv, Vol. 72 No. 6, 2002.
Izvorni znanstveni članak
A simple enzyme-linked immunosorbent assay for the detection of Aeromonas spp.
Neelam Sachan
; FAO/WHO Collaborating Centre for Research and Training in Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, India
Rajesh Kumar Agarwal
; FAO/WHO Collaborating Centre for Research and Training in Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, India
Sažetak
An indirect enzyme-linked immunosorbent assay employing antisera against somatic antigen and outer membrane protein of a rough Aeromonas strain was developed for the detection of Aeromonas spp. The test detected Aeromonas irrespective of its species or serotype. The optimum detection level was found to be 106/ml. The cross-reactions were observed only with Vibrio cholerae (O39) among the 15 different organisms belonging to Enterobacteriaceae, Vibrionaceae and a few Gram-positive pathogens tested. Preliminary trials on food samples revealed the test to be efficient in detecting Aeromonas spp.
Ključne riječi
Aeromonas spp.; ELISA; outer membrane protein; food
Hrčak ID:
81605
URI
Datum izdavanja:
18.12.2002.
Posjeta: 1.035 *