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GLIADIN LOCI COMPOSITION ANALYSIS OF CROATIAN HEXAPLOID WHEAT GERMPLASM

Ivana Rukavina ; Hrvatski centar za poljoprivredu,hranu i selo - Zavod za sjemenarstvo i rasadničarstvo
Sonja Marić ; Poljoprivredni fakultet Sveučilišta u Osijeku
Vlado Guberac ; Poljoprivredni fakultet Sveučilišta u Osijeku
Sonja Petrović ; Poljoprivredni fakultet Sveučilišta u Osijeku
Tihomir Čupić ; Poljoprivredni institut Osijek
Cornelia Tepper ; Bundessortenamt, Hannover, Njemačka

Sažetak

The composition of ω – gliadins was identified and analyzed in 50 varieties of
hexaploid winter wheat (Triticum aestivum ssp. vulgare L.) originated from the
Republic of Croatia. Polyacrylamide gel electrophoresis (PAGE) in presence of sodium
dodecyl sulfate (SDS) was used for determination of subunits at Gli-B1 i Gli-D1
genloci. The most common combination of subunits at genloci Gli-B1 was 63+67 with
frequency of 64%, then subunit 66 with frequency of 16% and null allele (N) with
frequency of 14%. At Gli-D1 loci the most frequent subunit was 55 with presence at
94% in tested varieties. Number of allels per loci was from 3 (Gli-D1) to 7 (Gli-B1),
high genetic diversity (He = 0,557) and high PIC (0,529) were estimated at loci Gli-B1,
while significantly lower genetic diversity was estimated at loci Gli-D1 (He = 0,114)
and PIC (0,110).

Ključne riječi

hexaploid wheat; variety; ω – gliadins; subunits; SDS PAGE

Hrčak ID:

103343

URI

https://hrcak.srce.hr/103343

Datum izdavanja:

28.3.2013.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.676 *