Izvorni znanstveni članak
The use of Molecular Markers to Determine Wheat Lines with Chasmogamic Type of Flowering
Danuta Kurasiak-Popowska
; Department of Plant Genetics and Breeding, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland
Zbigniew Broda
; Department of Plant Genetics and Breeding, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland
Piotr Tokarski
; Department of Plant Genetics and Breeding, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland
Jan Bocianowski
; Department of Mathematical and Statistical Methods, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland
Sažetak
Results of the genetic distance between two cultivars and seven lines of spring wheat are presented. Another objective of the presented paper was to find an amplification product that could be a marker of a faster growth stage and of the type of flowering. Random amplified polymorphic DNA (RAPD) was used in the studies. Forty selected primers were tested. Fourteen primers were used to develop a dendrogram presenting similarities among the cultivars and lines. Numerical analysis showed two similarity groups. The first group included KOH 5560, KOH 5637, KOH 5674, KOH 5884, KOH 5930 as well as ‚Nawara’ and ‚Zadra’. The second group consisted of: KOH 5561 and KOH 5718. Results showed the presence of some specific amplification products: OPA 09f, OPF 13k, OPF 14j, OPC 11k in wheat lines KOH 5884 and partially in KOH 5561 which indicate a fast rate of growth and chasmogamic type of flowering.
Ključne riječi
RAPD markers; flowering; spring wheat; association
Hrčak ID:
117940
URI
Datum izdavanja:
15.1.2014.
Posjeta: 766 *