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Some important aspects of implementing tandem mass spectrometry in a routine clinical laboratory environment

Christoph Seger
Andrea Griesmacher


Puni tekst: hrvatski pdf 691 Kb

str. 29-51

preuzimanja: 913

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Puni tekst: engleski pdf 691 Kb

str. 29-51

preuzimanja: 534

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Sažetak

During past years, the combination of high performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS), also known as HPLC-MS/MS, matured to reliable analytical instrumentation. Its application in therapeutic drug monitoring (TDM) has been shown to be superior to normally applied immunoassays. Especially in the context of immunosuppressant medication level surveillance, this technique has become an indispensable tool in many clinical laboratories and is considered the gold standard. However, setting up a HPLC-MS/MS platform is demanding in terms of assay validation and the robustness of testing. This review is to provide a rough guideline aiding the implementation process of such a platform into a routine clinical environment.

Ključne riječi

tandem mass spectrometry; targeted analysis; therapeutic drug monitoring; routine analysis; high performance liquid chromatography; HPLC-MS/MS

Hrčak ID:

12858

URI

https://hrcak.srce.hr/12858

Datum izdavanja:

7.6.2007.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.910 *