Izvorni znanstveni članak

Structural and ultrastructural evaluations of zebrafish ovaries after exposure to 2, 3, 7, 8 - Tetrachlorodibenzo-p-dioxin (TCDD)

Noelia PATRIGNANI ; Department of Comparative Anatomy and Pathological Anatomy, Veterinary Faculty, University of Córdoba, 14071 Córdoba, Spain
Ana MOLINA ; Department of Pharmacology, Toxicology and Legal and Forensic Medicine, Veterinary Faculty, University of Córdoba,14071 Córdoba, Spain
Alfonso BLANCO ; Department of Comparative Anatomy and Pathological Anatomy, Veterinary Faculty, University of Córdoba, 14071 Córdoba, Spain
María AYALA ; Department of Comparative Anatomy and Pathological Anatomy, Veterinary Faculty, University of Murcia, 30100 Murcia, Spain
Rosario MOYANO SALVAGO ; Department of Pharmacology, Toxicology and Legal and Forensic Medicine, Veterinary Faculty, University of Córdoba,14071 Córdoba, Spain

Sažetak

The morphological consequences of long-term exposure to low doses of 2, 3, 7, 8-tetrachlo rodibenzo-p-dioxin in the ovaries were nvestigated in 50 adult female zebrafish at structural and ultrastructural levels. Animals were exposed to graded concentrations of 2, 3, 7, 8-tetrachlorodiben zo-p-dioxin (10, 40, 100 and 270 ppb) for 21 days, then zebrafish were sacrificed by an overdose of anaesthetic solution tricaine methanesulfonate, and immediately samples were taken for morphological evaluation. At lower concentrations of exposure there was no evidence of morphological modifications, while at higher concentrations (100 and 270 ppb) we frequently observed degeneration and inflammation. Significant increases in follicular atresia were observed among all groups (p<0.05). These results indicate that long-term exposure to low doses of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin are able to induce morphological damage on the ovaries, which could then produce adverse effects on fish reproductive health.

Ključne riječi

TCDD; zebrafish; ovaries; histopathology; follicular atresia

Hrčak ID:

134953

URI

https://hrcak.srce.hr/134953

Datum izdavanja:

1.7.2014.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.179 *