Veterinarski arhiv, Vol. 85 No. 1, 2015.
Izvorni znanstveni članak
Use of the bubaline acellular diaphragm matrix for umbilical hernioplasty in pigs.
Vineet Kumar
orcid.org/0000-0001-7054-5987
; Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Junagadh, Gujarat, India; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India
Anil K. Gangwar
; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India
Naveen Kumar
; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India
Himani Singh
; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India
Sažetak
Acellularization of xenogenic biomaterials has been highly desired for implantation without considerable adverse inflammatory and immune responses. Therefore, the present study was undertaken to acellularize the diaphragm of buffalo origin and to evaluate the effi cacy of this prepared acellular diaphragm matrix (ADM) scaffolds for the repair of umbilical hernia in pigs. Buffalo diaphragms were acellularized with different concentrations of sodium deoxycholate over a range of periods. Produced ADM scaffolds were used during umbilical hernioplasty in 12 crossbred Landrace pigs. Harvested serum samples on post-implantation days (0, 15 and 30) were subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) to observe serum protein distribution pattern. Treatment with 2 % sodium deoxycholate lead to complete acellularization of the bubaline diaphragm at 48 h. Microscopic examination of the detergent treated matrix confirmed complete extraction of stromal cells and orderly arranged collagen fibres. All the hernias repaired with ADM remained sound over a period of 3 months. The serum protein distribution on post-implantation day 15 was found different from day zero and becomes normal on day 30. All the hernias implanted with ADM healed completely without graft rejections. The present study suggested that 2 % sodium deoxycholate can be used for complete acellularization of bubaline diaphragm, and produced ADM may be used safely for umbilical hernioplasty in pigs.
Ključne riječi
acellular diaphragm matrix; buffalo; pigs; SDS-PAGE; umbilical hernia
Hrčak ID:
135267
URI
Datum izdavanja:
23.2.2015.
Posjeta: 1.136 *