Acta clinica Croatica, Vol. 45 No. 3, 2006.
Izvorni znanstveni članak
Stromal Reaction in Synchronous in Situ and Invasive Urothelial Carcinoma of the Bladder
Ante Reljić
Davor Tomas
Tereza Gabelić
Jelena Zarubica
Stela Bulimbašić
Hana Fazlić
Hrvoje Čupić
Božo Krušlin
Sažetak
The aim was to investigate stromal reaction, including inflammation and stromal desmoplasia in in situ and invasive urothelial carcinoma of urinary bladder and to determine the possible value of reactive stromal changes in the diagnosis of lamina propria invasion. We analyzed specimens from 24 consecutive patients with synchronous in situ and invasive urothelial carcinoma in the same biopsy. Specimens were obtained by transurethral resection, fixed and routinely stained with H&E and Mallory method. Immunohistochemistry was performed by monoclonal antibodies to vimentin, smooth muscle actin and desmin. The intensity of immunostaining was graded semiquantitatively on a scale of 0-3, and expressed as 0 = 0%; 1 = up to 33%; 2 = more than 33% to 66%; and 3 = more than 66% of positive stromal cells. The intensity of inflammation was labeled as 0 = no inflammation, 1 = weak, 2 = moderate, and 3 = dense inflammatory reaction. Mallory trichrome method showed predominantly no staining or weak green staining in 14/24 invasive and 20/24 in situ urothelial carcinomas (p>0.05). There was statistically significantly increased vimentin and smooth muscle actin immunostaining in the stroma of invasive carcinoma as compared with in situ carcinoma (p<0.05). Inflammatory reaction was statistically stronger in invasive carcinoma (p<0.05). The immunohistochemical expression of myofibroblastic markers was significantly stronger in invasive urothelial carcinoma. This may aid in the diagnosis of lamina propria invasion in urothelial carcinoma of urinary bladder.
Ključne riječi
Bladder neoplasms - pathology; Bladder neoplasms - diagnosis; Urothelium - pathology; Urologic neoplasms - classification; Stromal cells - pathology
Hrčak ID:
14059
URI
Datum izdavanja:
1.9.2006.
Posjeta: 2.083 *