Izvorni znanstveni članak

Biosynthesis of Penicilium funiculosum 515 Cellulases on Cellulosic Wastes

Antea Kočulanin ; Hrvatska
Ivka Biočić ; Hrvatska
Zlata Jurić ; Hrvatska

Sažetak

Surface — culture conditions affecting the biosynthesis of cellulolytic enzymes by Penicillium funiculosum 515 on different agricultural wastes such as wheat bran, wheat straw, corn stems and corn cobs were investigated.
P. funiculosum 515 was cultivated on moistened milled substrates. Every day for 7 to 9 days of cultivation the samples were taken, extracted with water and then filtered. Cellulolytic activity of the filtrates obtained was determined on cotton, filter paper (Mandels and Weber 1969) and carboxymethylcellulose (Verhovceva 1965). The sugars produced were measured according to Somogyi (Nelson 1944).
Conditions for preparing inoculum were evaluated: the use of the conidia grown on malt agar or wheat bran, the use of mycelial inoculum and media composition for inocula cultivation. The highest cellulase
yield was achieved when mycelia grown in media containing salts according to Reese et al. (1950), 0.5#/o peptone, 0,l°/o Tween 80 (Mandels et al. 1975) and l°/o cellulosic substrate were used as inocula.
The salts composition used along with water for moistening media were examined and it was found that the composition according to Reese et al. (1950) was the optimal one.
Depending on basic substrate in media the different ratio and degrees of cellulolytic activities of single components were obtained. The maximum time for every single component synthesis of cellulase complex depended on the composition of cultivation media.
P. funiculosum 515 was able to grow on all tested cellulosic wastes and to produce cellulolytic enzymes as well.

Ključne riječi

Hrčak ID:

158208

URI

https://hrcak.srce.hr/158208

Datum izdavanja:

31.12.1977.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.224 *